High-speed electro-fusion and electro-transfection of plant protoplasts by a continuous flow electro-manipulator View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-05

AUTHORS

T. Hibi, H. Kano, M. Sugiura, T. Kazami, S. Kimura

ABSTRACT

A continuous flow electro-manipulator available both for mass production of fused and of transfected plant protoplasts was devised using a flow chamber with gold-coated glass panel electrodes. Up to 100 ml of protoplasts suspension were treated within 20 min at the rate of approximately 1×106 protoplasts / min. The yield of diheterokaryons between tobacco mesophyll and carrot root protoplasts reached approximately 10 % of total protoplasts by flow electro-fusion. More than 95 % of tobacco and cowpea mesophyll protoplasts became infected with tobacco mosaic virus RNA by flow electro-transfection. More... »

PAGES

153-157

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269311

DOI

http://dx.doi.org/10.1007/bf00269311

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039325826

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24241587


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