Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max L. Merr View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1984-12

AUTHORS

Bärbel Lippmann, Gunter Lippmann

ABSTRACT

A method for the induction of somatic embryos in soybean tissue cultures is described. Cotyledons from immature embryos were utilized as explant source. Supplementing the culture medium with auxins (2,4-D, MCPA, 2,4,5-T, NAA, IAA, IBA) caused formation of meristematic tissue on cotyledon explants. The extent of meristematic tissue formed depended on the kind and concentration of auxin in the culture medium. With 2,4-D and MCPA, embryoids originated from meristematic tissue. Embryoid formation rates were influenced by the developmental stage of the embryos serving as explant source and auxin concentration. Addition of cytokinins to the medium containing 2,4-D or supplementing it with high sugar concentrations inhibited the formation of meristematic tissue and of embryoids on cotyledon explants. More... »

PAGES

215-218

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269295

DOI

http://dx.doi.org/10.1007/bf00269295

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032709585

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24253569


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