Callus formation from protoplasts of a sugarbeet cell suspension culture View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-08

AUTHORS

László Szabados, Carina Gaggero

ABSTRACT

Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protoplast division was also considerably stimulated by the addition of 250 mg/l casein hydrolysate, 200 mg/l yeast extract, and 20% v/v conditioned culture medium to the protoplast culture medium. The highest division rate (up to 35% of the protoplasts) was achieved at a density of 4×10(4)- 1×10(5) protoplasts/ml. From the colonies callus and suspension cultures were readily obtained. More... »

PAGES

195-198

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269287

DOI

http://dx.doi.org/10.1007/bf00269287

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002663221

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24253880


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