Plant regeneration by organogenesis in Glycine max View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-04

AUTHORS

M. S. Wright, S. M. Koehler, M. A. Hinchee, M. G. Carnes

ABSTRACT

A procedure for the regeneration of fertile plants by organogenesis from tissue cultures of soybeans, Glycine max is described. Seeds were germinated on reduced inorganic salt MS medium containing 5μM BA. Cotyledonary nodes were excised and cultured on the same medium. Presence of BA in the medium during seed germination and culture of nodal explants was required for multiple shoot and shoot-bud formation. Histological analyses established the de novo nature of shoot regeneration. Separate reduction of the concentration of inorganic salts or substitution of sucrose for fructose during culture had minimal effects on the regeneration response. Conversely, if the BA was reduced, the inhibition response could not be overcome by increased salt concentration or altered carbon source. More... »

PAGES

150-154

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269257

DOI

http://dx.doi.org/10.1007/bf00269257

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014649252

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24248057


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