Mini-chromosomes: Plasmids which carry the E. coli replication origin View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-01

AUTHORS

W. Messer, H. E. N. Bergmans, M. Meijer, J. E. Womack, F. G. Hansen, K. von Meyenburg

ABSTRACT

We have isolated plasmids by linking the 5.9 MD EcoRI fragment of E. coli that carries the origin of replication to an EcoRI fragment that carries an ampicillin resistance determinant, but lacks an origin of replication. 3 plasmids of this type, pOC1, pOC2, and pOC3, are described in detail in this report. Although the plasmids have some adverse effect on the growth properties of the host strain, their existence shows that two functioning chromosomal origins can coexist in one cell.Deletions generated from this type of plasmids allow an allocation of the origin of replication of E. coli within a DNA segment less than 0.4 MD in size. More... »

PAGES

269-275

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00268852

DOI

http://dx.doi.org/10.1007/bf00268852

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005050526

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/355833


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194 schema:name Abt. Trautner, Max-Planck-Institut für molekulare Genetik, D-1000, Berlin 33
195 rdf:type schema:Organization
 




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