Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1977-01

AUTHORS

Richard Meyer, David Figurski, Donald R. Helinski

ABSTRACT

The cleavage map of the plasmid RK2 was determined for the five restriction endonucleases EcoRI, HindIII, BamH-I, SalI and HpaI. DNA has been inserted into several of these sites and cloned in Escherichia coli. Efforts to obtain derivatives of RK2 reduced in size by restriction endonuclease digestion of the plasmid were not successful and indicated that genes required for the maintenance of this plasmid in E. coli are not tightly clustered. An RK2 derivative possessing an internal molecular rearrangement was obtained by transformation with restriction endonuclease digests of the plasmid. More... »

PAGES

129-135

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00268809

DOI

http://dx.doi.org/10.1007/bf00268809

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036352663

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/327269


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