Characterization of the cellulase complex from Cellulomonas grown on bagasse pith View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-06

AUTHORS

H. Rodríguez, O. Volfová, A. Klyosov

ABSTRACT

The effect of physico-chemical parameters on the cellulolytic activity of Cellulomonas sp. IIbc grown on sugarcane bagasse pith was investigated, and the optimum ranges for enzyme activity were established. The cellulases were more stable when incubated at the optimum growth temperature (32°C) than under optimum activity conditions (45°C for β-glucosidases and 50°C for CMC- and FP-cellulases). The β-glucosidases were the thermostability-limiting enzymes of the complex. Two types of endoglucanases could be recognized according to their adsorption properties on bagasse: one weakly-bound and one tightly-bound type, the latter constituting approximately 73% of the extracellular endoglucanases at exponential growth phase. Four forms active on filter paper and three active on CMC were obtained by HPLC separation of the extracellular fraction of the culture at stationary phase. More... »

PAGES

394-397

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00268202

DOI

http://dx.doi.org/10.1007/bf00268202

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038174306


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