Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-01

AUTHORS

N. P. Fiil, D. Bendiak, J. Collins, J. D. Friesen

ABSTRACT

Fragments of λdrifd 18 DNA with different end-points within the set of structural genes of ribosomal proteins L11 (rplK), L1 (rplA), L10 (rplJ) and L12 (rplL) as well as the β (rpoB) and β′ (rpoC) subunits of RNA polymerase have been cloned on plasmids. These plasmids were transformed in host cells which were mutant for each of the genes, enabling expression of both wild-type (plasmid-borne) and mutant (chromosomal) genes to be differentiated. On the basis of these results we propose the following genetic structure for the region: rplK and rplA are in one operon; rplL, rpoB and rpoC are in a second. Our data suggest the possibility that rplJ is by itself in an operon situated between the other two. More... »

PAGES

39-50

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00267689

DOI

http://dx.doi.org/10.1007/bf00267689

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016465137

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/381841


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