Transfection and transformation of Agrobacterium tumefaciens View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-01

AUTHORS

M. Holsters, D. de Waele, A. Depicker, E. Messens, M. van Montagu, J. Schell

ABSTRACT

The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation of A. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population. In transformation of the strain GV3100 with the P type plasmid RP4 a maximum frequency of 3.5 10(-7) transformants per total recipient population was obtained. Agrobacterium Ti-plasmids were introduced in the strain GV3100 with a maximal efficiency of 4.5 10(-8). These experiments provide further evidence that the Ti-plasmid is responsible for the oncogenic properties of A tumefaciens and for its capacity to induce "opine" synthesis in Crown-gall plant cells. More... »

PAGES

181-187

Journal

TITLE

Molecular Genetics and Genomics

ISSUE

2

VOLUME

163

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00267408

    DOI

    http://dx.doi.org/10.1007/bf00267408

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/355847


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