A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-12

AUTHORS

Richard A. Jorgensen, Steven J. Rothstein, Williams S. Reznikoff

ABSTRACT

This paper reports a cleavage site map of Tn5 for restriction enzymes BamHI, Bg/I, Bg/II, Hind II, HindIII, HpaI, Sa/I, Aval, SmaI, XhoI, PstI, PvuII, HaeII and HaeIII that was determined by the analysis of restriction enzyme cleavage patterns of ColEl, two independent ColEl::Tn5 plasmids, and a ColEl::Tn5 deletion derivative. Ba/I, EcoRI, KpnI, and PvuI do not cleave Tn5. Construction and analysis of in vitro-generated deletions of a ColEl::Tn5 plasmid limit the sequences encoding neomycin resistance to a 1500-base-pair-long segment of Tn5. Insertion of DNA at a Bg/II site within this segment results in loss of the neomycin resistance phenotype. Since this Bg/II site lies in an inverted repeat region, sequences within this repeat seem to be involved in the expression of neomycin resistance. More... »

PAGES

65-72

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00267254

DOI

http://dx.doi.org/10.1007/bf00267254

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000633250

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/231729


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