Callus formation from stem protoplasts of corn (Zea mays L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1977-01

AUTHORS

I. Potrykus, C. T. Harms, H. Lörz, E. Thomas

ABSTRACT

The first successful culture, with sustained divisions, of protoplasts from intact plants of Zea mays is described. The method involves the use of a hanging microdrop array technique which permits the testing of very large numbers of different culture media and hormone variations. Several different phytohormone combinations were found to allow sustained divisions in protoplasts isolated from stem tissue of corn plants, suggesting the importance of the source of the protoplasts rather than specific medium conditions. In some cases more than 5% of the protoplasts divided, giving macroscopic calluses within 35 days. More... »

PAGES

347-350

Journal

TITLE

Molecular Genetics and Genomics

ISSUE

3

VOLUME

156

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00267191

    DOI

    http://dx.doi.org/10.1007/bf00267191

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1031151606


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