Proteins occurring at, or near, the subunit interface of E. coli ribosomes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1973-12

AUTHORS

C. A. Morrison, R. A. Garrett, H. Zeichhardt, G. Stöffler

ABSTRACT

The identification of ribosomal proteins that occur at, or near, the subunit interface of the 30S and 50S subunits in the E. coli 70S ribosome was attempted by studying the effect of antibodies on the Mg++ dependent dissociation-association equilibrium of 70S ribosomes. Dissociated ribosomes were mixed with monovalent fragments of IgG antibodies (Fab's) specific for each ribosomal protein and then reassociated into intact 70S particles. Various degrees of inhibition of this reassociation were observed for proteins S9, S11, S12, S14, S20, L1, L6, L14, L15, L19, L20, L23, L26 and L27. A small amount of aggregation of 50S subunits was caused by IgG's specific for the proteins S9, S11, S12, S14 and S20 and purified 50S subunits. It was inferred that the presence of small amounts of these proteins on 50S subunits was compatible with their presence at the subunit interface. Finally, the capacity of proteins S11 and S12 to bind to 23S RNA was demonstrated. More... »

PAGES

359-368

References to SciGraph publications

  • 1972-06. Ribosomal proteins in MOLECULAR GENETICS AND GENOMICS
  • 1973-06. The accessibility of proteins of the Escherichia coli 30S ribosomal subunit to antibody binding in MOLECULAR GENETICS AND GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00267106

    DOI

    http://dx.doi.org/10.1007/bf00267106

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011456996

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/4594008


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