Fusion of plant protoplasts: a study using auxotrophic mutants of Nicotiana plumbaginifolia, Viviani View Full Text


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Article Info

DATE

1986-03

AUTHORS

I. Negrutiu, D. De Brouwer, J. W. Watts, V. I. Sidorov, R. Dirks, M. Jacobs

ABSTRACT

Protoplast fusion studies between various auxotrophic mutants of Nicotiana plumbaginifolia were performed to optimize conditions for PEG-mediated fusion and to identify factors influencing the plant protoplast fusion process. Numerous parameters in the isolation, culture, and fusion of protoplasts were tested, and established fusion protocols were compared. Fusion rates, calculated on the basis of colony growth on selection medium (genetic complementation), ranged from 10(-4) to 10(-2). Conditions that allow rapid and reproducible fusions at the highest rates were established. Particular emphasis was given to fusion of mesophyll-derived protoplasts, for which the ability to regenerate fertile plants from fusion products was shown to be particularly high. Preliminary experiments using electric-field mediated fusion suggest that electrofusion may offer significant advantages over the traditional chemical fusion. More... »

PAGES

279-286

References to SciGraph publications

  • 1974-12. A method for high-frequency intergeneric fusion of plant protoplasts in PLANTA
  • 1974-01. Plant protoplast fusion and growth of intergeneric hybrid cells in PLANTA
  • 1983-09. Regeneration of fully nitrate reductase — deficient mutants from protoplast culture of Nicotiana plumbaginifolia (Viviani) in THEORETICAL AND APPLIED GENETICS
  • 1978-01. Chemical inhibition of cell wall formation and cytokinesis, but not of nuclear division, in protoplasts of Nicotiana tabacum L. cultivated in vitro in PLANTA
  • 1976-10. Somatic hybridisation of Petunia hybrida and P. parodii in NATURE
  • 1979-05. Use of amino acid analogue-resistant cell lines for selection of Nicotiana sylvestris somatic cell hybrids in THEORETICAL AND APPLIED GENETICS
  • 1981-01. High frequency fusion of plant protoplasts by electric fields in PLANTA
  • 1981-03. Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids in THEORETICAL AND APPLIED GENETICS
  • 1983. Carrot Ⓧ Tobacco Somatic Cell Hybrids Selected by Amino Acid Analog Resistance Complementation in PROTOPLASTS 1983
  • 1983-01. Culture and selection of somatic hybrids using an auxotrophic cell line in THEORETICAL AND APPLIED GENETICS
  • 1984-09. Progress in cellular engineering of plants: biochemical and genetic assessment of selectable markers from cultured cells in PLANT MOLECULAR BIOLOGY
  • 1982-10. Protoplast fusion and the cell cycle in PLANT CELL REPORTS
  • 1985-05. Amino acid auxotrophs from protoplast cultures of Nicotiana plumbaginifolia, Viviani in MOLECULAR GENETICS AND GENOMICS
  • 1983-10. Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species in PLANT CELL REPORTS
  • 1984-04. A simple method for large-scale electrofusion and culture of plant protoplasts in BIOSCIENCE REPORTS
  • 1984. Protoplast Fusion in NONE
  • 1982-09. Nitrate reductase deficient cell lines from haploid protoplast cultures ofNicotiana plumbaginifolia in MOLECULAR GENETICS AND GENOMICS
  • 1985-11. Complementation analysis by somatic hybridisation and genetic crosses of nitrate reductase-deficient mutants of Nicotiana plumbaginifolia in MOLECULAR GENETICS AND GENOMICS
  • 1985-06. Culture of plant somatic hybrids following electrical fusion in THEORETICAL AND APPLIED GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00267005

    DOI

    http://dx.doi.org/10.1007/bf00267005

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24247847


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    34 schema:description Protoplast fusion studies between various auxotrophic mutants of Nicotiana plumbaginifolia were performed to optimize conditions for PEG-mediated fusion and to identify factors influencing the plant protoplast fusion process. Numerous parameters in the isolation, culture, and fusion of protoplasts were tested, and established fusion protocols were compared. Fusion rates, calculated on the basis of colony growth on selection medium (genetic complementation), ranged from 10(-4) to 10(-2). Conditions that allow rapid and reproducible fusions at the highest rates were established. Particular emphasis was given to fusion of mesophyll-derived protoplasts, for which the ability to regenerate fertile plants from fusion products was shown to be particularly high. Preliminary experiments using electric-field mediated fusion suggest that electrofusion may offer significant advantages over the traditional chemical fusion.
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