Histochemical studies of pollen: storage pockets in the endoplasmic reticulum (ER) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1968-06

AUTHORS

Donald B. Fisher, William A. Jensen, Mary E. Ashton

ABSTRACT

The pollen grain of cotton (Gossypium hirsutum) was examined histochemically at the light and electron microscope level. The cytoplasm of the pollen contains an unusual storage unit which consists of a pocket of endoplasmic reticulum (ER) containing lipid droplets and dictyosome vesicles. The ER pockets are large enough to be seen with the light microscope if thin enough sections are used (0.3–1.5μ). The results of the histochemical analyses show that the dictysome vesicles are rich in carbohydrate and contain protein and lipid as well. The ER contains large amounts of protein which may be arginine rich. Some carbohydrates may also be present in the ER. The ER is covered with ribosomes so that the pockets are unusually rich storage units containing abundant protein, carbohydrate, lipid and RNA. The light microscope localization of carbohydrates was confirmed by the periodic acid-silver method. Other storage units in the cytoplasm were also studied. A new method for the embedding of plant tissue for thin sectioning for light microscopy is presented. More... »

PAGES

169-182

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00266578

DOI

http://dx.doi.org/10.1007/bf00266578

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013599047

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/5741845


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Acetone", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alkanes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aniline Compounds", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carbohydrates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endoplasmic Reticulum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Freezing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gossypium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Histocytochemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lipids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Electron", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pollen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Povidone", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of California, Berkeley", 
          "id": "https://www.grid.ac/institutes/grid.47840.3f", 
          "name": [
            "Department of Botany, University of California, 94720, Berkeley, California"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fisher", 
        "givenName": "Donald B.", 
        "id": "sg:person.07535400464.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07535400464.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, Berkeley", 
          "id": "https://www.grid.ac/institutes/grid.47840.3f", 
          "name": [
            "Department of Botany, University of California, 94720, Berkeley, California"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jensen", 
        "givenName": "William A.", 
        "id": "sg:person.0577104702.68", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577104702.68"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, Berkeley", 
          "id": "https://www.grid.ac/institutes/grid.47840.3f", 
          "name": [
            "Department of Botany, University of California, 94720, Berkeley, California"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ashton", 
        "givenName": "Mary E.", 
        "id": "sg:person.060616626.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.060616626.92"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0006-3002(63)91092-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011657194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-3002(63)91092-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011657194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/15.7.409", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026901674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/15.7.409", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026901674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/15.8.442", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043683068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/15.8.442", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043683068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/10520295209105092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051780035"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1968-06", 
    "datePublishedReg": "1968-06-01", 
    "description": "The pollen grain of cotton (Gossypium hirsutum) was examined histochemically at the light and electron microscope level. The cytoplasm of the pollen contains an unusual storage unit which consists of a pocket of endoplasmic reticulum (ER) containing lipid droplets and dictyosome vesicles. The ER pockets are large enough to be seen with the light microscope if thin enough sections are used (0.3\u20131.5\u03bc). The results of the histochemical analyses show that the dictysome vesicles are rich in carbohydrate and contain protein and lipid as well. The ER contains large amounts of protein which may be arginine rich. Some carbohydrates may also be present in the ER. The ER is covered with ribosomes so that the pockets are unusually rich storage units containing abundant protein, carbohydrate, lipid and RNA. The light microscope localization of carbohydrates was confirmed by the periodic acid-silver method. Other storage units in the cytoplasm were also studied. A new method for the embedding of plant tissue for thin sectioning for light microscopy is presented.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00266578", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1294817", 
        "issn": [
          "0948-6143", 
          "1432-119X"
        ], 
        "name": "Histochemistry and Cell Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "13"
      }
    ], 
    "name": "Histochemical studies of pollen: storage pockets in the endoplasmic reticulum (ER)", 
    "pagination": "169-182", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "40c88051c632f79714378c0787c8d07419769fa8696baede1aa46326544b4b49"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "5741845"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0411277"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00266578"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1013599047"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00266578", 
      "https://app.dimensions.ai/details/publication/pub.1013599047"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T14:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130830_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00266578"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00266578'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00266578'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00266578'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00266578'


 

This table displays all metadata directly associated to this object as RDF triples.

151 TRIPLES      21 PREDICATES      47 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00266578 schema:about N1504f32d691a47d4891becfa655acb71
2 N2211b16d75a342168586b4e2f7688811
3 N2807b06091f24c919a0aa01830b59dbd
4 N353e306df91544808983a0b081a8b0e7
5 N38bd1ba03f544f14ad3f0787c148f7e8
6 N3fffe5266e5441cdba8ee3046b39c262
7 N53ad4365853149c1875c1fd9c4be99cf
8 Naa044369de4f4f32b62ac1cb6f5e0675
9 Nae1a3c22e24c4004850c0107342eb832
10 Nc577f5af9c5341e697cfb657c04aa654
11 Nc9eb06168dd04df19937408bab92fd1d
12 Nd168664a679c4f4caa088a05fcd6faf6
13 Ndaaef3a5c8d1408a96e33e6b173aec7a
14 Nf881e4409f4b44589a9e9fb3a00fc4d6
15 anzsrc-for:06
16 anzsrc-for:0601
17 schema:author N60a92cbeadbd4e958e9019dbce5b811a
18 schema:citation https://doi.org/10.1016/0006-3002(63)91092-8
19 https://doi.org/10.1177/15.7.409
20 https://doi.org/10.1177/15.8.442
21 https://doi.org/10.3109/10520295209105092
22 schema:datePublished 1968-06
23 schema:datePublishedReg 1968-06-01
24 schema:description The pollen grain of cotton (Gossypium hirsutum) was examined histochemically at the light and electron microscope level. The cytoplasm of the pollen contains an unusual storage unit which consists of a pocket of endoplasmic reticulum (ER) containing lipid droplets and dictyosome vesicles. The ER pockets are large enough to be seen with the light microscope if thin enough sections are used (0.3–1.5μ). The results of the histochemical analyses show that the dictysome vesicles are rich in carbohydrate and contain protein and lipid as well. The ER contains large amounts of protein which may be arginine rich. Some carbohydrates may also be present in the ER. The ER is covered with ribosomes so that the pockets are unusually rich storage units containing abundant protein, carbohydrate, lipid and RNA. The light microscope localization of carbohydrates was confirmed by the periodic acid-silver method. Other storage units in the cytoplasm were also studied. A new method for the embedding of plant tissue for thin sectioning for light microscopy is presented.
25 schema:genre research_article
26 schema:inLanguage en
27 schema:isAccessibleForFree false
28 schema:isPartOf Ne0ea78772a8041f6905215e1322a74ac
29 Nedc3e9893bc24c858d24589b23abbe2c
30 sg:journal.1294817
31 schema:name Histochemical studies of pollen: storage pockets in the endoplasmic reticulum (ER)
32 schema:pagination 169-182
33 schema:productId N38b03e02f63c4cb6a575bd110dd29a36
34 N6bddcb79a41e440b9738abddb4eff83e
35 Na98df7a610ac400a98aad6a3877bbfe6
36 Nae24e5563711425db5ecba6b1acad7d7
37 Nec0e17eecc9640d682adcb4f9bdc2ee0
38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013599047
39 https://doi.org/10.1007/bf00266578
40 schema:sdDatePublished 2019-04-11T14:01
41 schema:sdLicense https://scigraph.springernature.com/explorer/license/
42 schema:sdPublisher N341d938336eb42809e462e09a44780ce
43 schema:url http://link.springer.com/10.1007/BF00266578
44 sgo:license sg:explorer/license/
45 sgo:sdDataset articles
46 rdf:type schema:ScholarlyArticle
47 N1504f32d691a47d4891becfa655acb71 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Histocytochemistry
49 rdf:type schema:DefinedTerm
50 N2211b16d75a342168586b4e2f7688811 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
51 schema:name RNA
52 rdf:type schema:DefinedTerm
53 N2807b06091f24c919a0aa01830b59dbd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
54 schema:name Plant Proteins
55 rdf:type schema:DefinedTerm
56 N341d938336eb42809e462e09a44780ce schema:name Springer Nature - SN SciGraph project
57 rdf:type schema:Organization
58 N353e306df91544808983a0b081a8b0e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
59 schema:name Povidone
60 rdf:type schema:DefinedTerm
61 N38b03e02f63c4cb6a575bd110dd29a36 schema:name dimensions_id
62 schema:value pub.1013599047
63 rdf:type schema:PropertyValue
64 N38bd1ba03f544f14ad3f0787c148f7e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name Endoplasmic Reticulum
66 rdf:type schema:DefinedTerm
67 N3fffe5266e5441cdba8ee3046b39c262 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Alkanes
69 rdf:type schema:DefinedTerm
70 N53ad4365853149c1875c1fd9c4be99cf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Freezing
72 rdf:type schema:DefinedTerm
73 N607f44eca36f4905b82b3d06f8d7dc83 rdf:first sg:person.060616626.92
74 rdf:rest rdf:nil
75 N60a92cbeadbd4e958e9019dbce5b811a rdf:first sg:person.07535400464.32
76 rdf:rest Na2abfd67ab024d578188eed857daf0e2
77 N6bddcb79a41e440b9738abddb4eff83e schema:name doi
78 schema:value 10.1007/bf00266578
79 rdf:type schema:PropertyValue
80 Na2abfd67ab024d578188eed857daf0e2 rdf:first sg:person.0577104702.68
81 rdf:rest N607f44eca36f4905b82b3d06f8d7dc83
82 Na98df7a610ac400a98aad6a3877bbfe6 schema:name pubmed_id
83 schema:value 5741845
84 rdf:type schema:PropertyValue
85 Naa044369de4f4f32b62ac1cb6f5e0675 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Lipids
87 rdf:type schema:DefinedTerm
88 Nae1a3c22e24c4004850c0107342eb832 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Pollen
90 rdf:type schema:DefinedTerm
91 Nae24e5563711425db5ecba6b1acad7d7 schema:name readcube_id
92 schema:value 40c88051c632f79714378c0787c8d07419769fa8696baede1aa46326544b4b49
93 rdf:type schema:PropertyValue
94 Nc577f5af9c5341e697cfb657c04aa654 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Microscopy, Electron
96 rdf:type schema:DefinedTerm
97 Nc9eb06168dd04df19937408bab92fd1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Carbohydrates
99 rdf:type schema:DefinedTerm
100 Nd168664a679c4f4caa088a05fcd6faf6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Aniline Compounds
102 rdf:type schema:DefinedTerm
103 Ndaaef3a5c8d1408a96e33e6b173aec7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Gossypium
105 rdf:type schema:DefinedTerm
106 Ne0ea78772a8041f6905215e1322a74ac schema:volumeNumber 13
107 rdf:type schema:PublicationVolume
108 Nec0e17eecc9640d682adcb4f9bdc2ee0 schema:name nlm_unique_id
109 schema:value 0411277
110 rdf:type schema:PropertyValue
111 Nedc3e9893bc24c858d24589b23abbe2c schema:issueNumber 2
112 rdf:type schema:PublicationIssue
113 Nf881e4409f4b44589a9e9fb3a00fc4d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Acetone
115 rdf:type schema:DefinedTerm
116 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
117 schema:name Biological Sciences
118 rdf:type schema:DefinedTerm
119 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
120 schema:name Biochemistry and Cell Biology
121 rdf:type schema:DefinedTerm
122 sg:journal.1294817 schema:issn 0948-6143
123 1432-119X
124 schema:name Histochemistry and Cell Biology
125 rdf:type schema:Periodical
126 sg:person.0577104702.68 schema:affiliation https://www.grid.ac/institutes/grid.47840.3f
127 schema:familyName Jensen
128 schema:givenName William A.
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577104702.68
130 rdf:type schema:Person
131 sg:person.060616626.92 schema:affiliation https://www.grid.ac/institutes/grid.47840.3f
132 schema:familyName Ashton
133 schema:givenName Mary E.
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.060616626.92
135 rdf:type schema:Person
136 sg:person.07535400464.32 schema:affiliation https://www.grid.ac/institutes/grid.47840.3f
137 schema:familyName Fisher
138 schema:givenName Donald B.
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07535400464.32
140 rdf:type schema:Person
141 https://doi.org/10.1016/0006-3002(63)91092-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011657194
142 rdf:type schema:CreativeWork
143 https://doi.org/10.1177/15.7.409 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026901674
144 rdf:type schema:CreativeWork
145 https://doi.org/10.1177/15.8.442 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043683068
146 rdf:type schema:CreativeWork
147 https://doi.org/10.3109/10520295209105092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051780035
148 rdf:type schema:CreativeWork
149 https://www.grid.ac/institutes/grid.47840.3f schema:alternateName University of California, Berkeley
150 schema:name Department of Botany, University of California, 94720, Berkeley, California
151 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...