Histochemical studies of pollen: storage pockets in the endoplasmic reticulum (ER) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1968-06

AUTHORS

Donald B. Fisher, William A. Jensen, Mary E. Ashton

ABSTRACT

The pollen grain of cotton (Gossypium hirsutum) was examined histochemically at the light and electron microscope level. The cytoplasm of the pollen contains an unusual storage unit which consists of a pocket of endoplasmic reticulum (ER) containing lipid droplets and dictyosome vesicles. The ER pockets are large enough to be seen with the light microscope if thin enough sections are used (0.3–1.5μ). The results of the histochemical analyses show that the dictysome vesicles are rich in carbohydrate and contain protein and lipid as well. The ER contains large amounts of protein which may be arginine rich. Some carbohydrates may also be present in the ER. The ER is covered with ribosomes so that the pockets are unusually rich storage units containing abundant protein, carbohydrate, lipid and RNA. The light microscope localization of carbohydrates was confirmed by the periodic acid-silver method. Other storage units in the cytoplasm were also studied. A new method for the embedding of plant tissue for thin sectioning for light microscopy is presented. More... »

PAGES

169-182

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00266578

DOI

http://dx.doi.org/10.1007/bf00266578

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013599047

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/5741845


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