Transformation of Brassica napus L. using Agrobacterium tumefaciens: developmentally regulated expression of a reintroduced napin gene View Full Text


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Article Info

DATE

1988-05

AUTHORS

S. E. Radke, B. M. Andrews, M. M. Moloney, M. L. Crouch, J. C. Kridl, V. C. Knauf

ABSTRACT

Genetically transformed plants of Brassica napus L. (oilseed rape) were obtained from hypocotyl expiants using Agrobacterium tumefaciens vectors. Hypocotyl explants were inoculated with disarmed or oncogenic A. tumefaciens strains, EHA101 and A281, and then cultured on media containing kanamycin. The A. tumefaciens strains harbored a binary vector, which contained a neomycin phosphotransferase II (NPTII) gene driven by the 35S promoter of cauliflower mosaic virus and an engineered napin (seed storage protein) gene with its own promoter (300 nucleotides 5′ to the start of translation). Transformation of B. napus plants was confirmed by detection of NPT II enzyme activity, Southern blot analysis and inheritance of the kanamycin-resistance trait (NPT II gene) in the progeny. Expression of the engineered napin gene in embryos but not in leaves of transgenic plants was observed by Northern analysis. These data demonstrate that morphologically normal, fertile transgenic B. napus plants can be obtained using Agrobacterium as a gene vector and that developmentally regulated expression of reintroduced genes can be achieved. More... »

PAGES

685-694

References to SciGraph publications

  • 1985-02. A simple culture method for Brassica hypototyl protoplasts in PLANT CELL REPORTS
  • 1986-04. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens in PLANT CELL REPORTS
  • 1987-07. Transgenic plants protected from insect attack in NATURE
  • 1981-05. DNA sequence of a plasmid-encoded dihydrofolate reductase in MOLECULAR GENETICS AND GENOMICS
  • 1979-01. Bioenergetic considerations in the improvement of oil content and quality in oil-seed crops in THEORETICAL AND APPLIED GENETICS
  • 1986-04. A rapid and efficient alternative procedure for the regeneration of plants from hypocotyl protoplasts of Brassica napus in PLANT CELL REPORTS
  • 1987-07. Efficient Transfer of a Glyphosate Tolerance Gene into Tomato Using a Binary Agrobacterium Tumefaciens Vector in NATURE BIOTECHNOLOGY
  • 1985-09. High frequency plant regeneration from thin cell layer explants of Brassica napus in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1986-07. Potato spindle tuber viroid infections mediated by the Ti plasmid of Agrobacterium tumefaciens in PLANT MOLECULAR BIOLOGY
  • 1985-12. Genetic manipulation in cultivars of oilseed rape (Brassica napus) using Agrobacterium in THEORETICAL AND APPLIED GENETICS
  • 1978-01. Transfection and transformation of Agrobacterium tumefaciens in MOLECULAR GENETICS AND GENOMICS
  • 1983-05. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid in NATURE
  • 1987-02. Agrobacterium mediated transformation and regeneration of Populus in MOLECULAR GENETICS AND GENOMICS
  • 1987-08. Transgenic Plants of Brassica napus L. in NATURE BIOTECHNOLOGY
  • 1987-03. Genetic transformation of oilseed rape (Brassica napus) by the Ri T-DNA of Agrobacterium rhizogenes and analysis of inheritance of the transformed phenotype in MOLECULAR GENETICS AND GENOMICS
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • 1985-10. Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate in NATURE
  • 1985-05. A simple culture method forBrassica hypocotyl protoplasts in PLANT CELL REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00265588

    DOI

    http://dx.doi.org/10.1007/bf00265588

    DIMENSIONS

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