Somatic embryogenesis and plant regeneration from leaf tissues and anthers of Pennisetum purpureum Schum. View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1981-09

AUTHORS

Z. Haydu, I. K. Vasil

ABSTRACT

Expiants obtained from leaves of Pennisetum purpureum Schum. gave rise to callus tissues when cultured on Murashige and Skoog's nutrient medium containing only 2,4-dichlorophenoxyacetic acid (2,4-D), or 2,4-D supplemented with naphthalene-acetic acid and/or 6-benzylaminopurine. Embryoids were formed in the white, compact and highly organised areas of the callus and developed into plants. Histological examination of cultured leaf segments showed that the callus tissue was formed by division in cells of the mesophyll tissue as well as the lower epidermis. A rapidly growing embryogenic callus tissue was also obtained apparently from the somatic tissues of anthers of P. purpureum, and induced to form plants through somatic embryogenesis. Plants obtained from leaf and anther culture were grown to maturity, and were shown to have the normal chromosome number of 2n = 4x = 28. More... »

PAGES

269-273

Journal

TITLE

Theoretical and Applied Genetics

ISSUE

5

VOLUME

59

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00264978

DOI

http://dx.doi.org/10.1007/bf00264978

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006664952

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24276510


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