Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1974-12

AUTHORS

Ian W. Dawes, Ian D. Hardie

ABSTRACT

Ascospores of Saccharomyces cerevisiae are considerably less sensitive to ether than vegetative yeast. Sporulated cultures grown on either liquid or solid media can be treated with ether to selectively kill vegetative organisms. This differential sensitivity of vegetative cells can be exploited to advantage in a number of genetic manipulations. These include: random spore analysis; selecting mutants affected in the developmental processes of sporulation and spore germination; detection of sporulating revertants or recombinants from non-sporulating diploids; determination of the incidence of spores present in cultures at frequencies as low as 1 per 106 viable organisms; and, complementation testing of developmental mutations. More... »

PAGES

281-289

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00264859

DOI

http://dx.doi.org/10.1007/bf00264859

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002106771

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4612332


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ethyl Ethers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spores, Fungal", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Edinburgh", 
          "id": "https://www.grid.ac/institutes/grid.4305.2", 
          "name": [
            "Department of Microbiology, University of Edinburgh, Edinburgh, Scotland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dawes", 
        "givenName": "Ian W.", 
        "id": "sg:person.01301763033.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301763033.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Edinburgh", 
          "id": "https://www.grid.ac/institutes/grid.4305.2", 
          "name": [
            "Department of Microbiology, University of Edinburgh, Edinburgh, Scotland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hardie", 
        "givenName": "Ian D.", 
        "id": "sg:person.076111243.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.076111243.63"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1515/znb-1958-1004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002140139"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/znb-1958-1004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002140139"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-2911(08)60252-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005151665"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1180667", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033290847"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1180667", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033290847"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.158.3802.803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062492637"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075782231", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076162524", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076495428", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077358086", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080455781", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080731252", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080833544", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081018870", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081351786", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1974-12", 
    "datePublishedReg": "1974-12-01", 
    "description": "Ascospores of Saccharomyces cerevisiae are considerably less sensitive to ether than vegetative yeast. Sporulated cultures grown on either liquid or solid media can be treated with ether to selectively kill vegetative organisms. This differential sensitivity of vegetative cells can be exploited to advantage in a number of genetic manipulations. These include: random spore analysis; selecting mutants affected in the developmental processes of sporulation and spore germination; detection of sporulating revertants or recombinants from non-sporulating diploids; determination of the incidence of spores present in cultures at frequencies as low as 1 per 106 viable organisms; and, complementation testing of developmental mutations.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00264859", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297380", 
        "issn": [
          "1617-4615", 
          "1432-1874"
        ], 
        "name": "Molecular Genetics and Genomics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "131"
      }
    ], 
    "name": "Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether", 
    "pagination": "281-289", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "0a089065e3b2697b2ad748f11ef780a5d7ff37ef1f4799f2802eb7086be240c3"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "4612332"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0125036"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00264859"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1002106771"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00264859", 
      "https://app.dimensions.ai/details/publication/pub.1002106771"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130794_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00264859"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00264859'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00264859'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00264859'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00264859'


 

This table displays all metadata directly associated to this object as RDF triples.

122 TRIPLES      21 PREDICATES      46 URIs      25 LITERALS      13 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00264859 schema:about N5f0373c1a3a541febddd72deea4f2947
2 N68c891f7b0bb4768b68c5625f7d864f4
3 N78f0c99f5b3946cdad0a1de273a017c0
4 Na1b4382bc8ec4f3abb5199c9811f4e66
5 anzsrc-for:06
6 anzsrc-for:0604
7 schema:author Nb70b2297c73342dfafbff6e0513d40be
8 schema:citation https://app.dimensions.ai/details/publication/pub.1075782231
9 https://app.dimensions.ai/details/publication/pub.1076162524
10 https://app.dimensions.ai/details/publication/pub.1076495428
11 https://app.dimensions.ai/details/publication/pub.1077358086
12 https://app.dimensions.ai/details/publication/pub.1080455781
13 https://app.dimensions.ai/details/publication/pub.1080731252
14 https://app.dimensions.ai/details/publication/pub.1080833544
15 https://app.dimensions.ai/details/publication/pub.1081018870
16 https://app.dimensions.ai/details/publication/pub.1081351786
17 https://doi.org/10.1016/s0065-2911(08)60252-0
18 https://doi.org/10.1042/bj1180667
19 https://doi.org/10.1126/science.158.3802.803
20 https://doi.org/10.1515/znb-1958-1004
21 schema:datePublished 1974-12
22 schema:datePublishedReg 1974-12-01
23 schema:description Ascospores of Saccharomyces cerevisiae are considerably less sensitive to ether than vegetative yeast. Sporulated cultures grown on either liquid or solid media can be treated with ether to selectively kill vegetative organisms. This differential sensitivity of vegetative cells can be exploited to advantage in a number of genetic manipulations. These include: random spore analysis; selecting mutants affected in the developmental processes of sporulation and spore germination; detection of sporulating revertants or recombinants from non-sporulating diploids; determination of the incidence of spores present in cultures at frequencies as low as 1 per 106 viable organisms; and, complementation testing of developmental mutations.
24 schema:genre research_article
25 schema:inLanguage en
26 schema:isAccessibleForFree false
27 schema:isPartOf N60f625de17444b97bea3c4239076d340
28 Ncf47902c0c45468a827fd49d3843b38a
29 sg:journal.1297380
30 schema:name Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether
31 schema:pagination 281-289
32 schema:productId N0b434600db2b4e0cb16fbc13135ce41a
33 N10da0508874e4d8999f7052f74948dcc
34 N3d43fd79c70b4205836ffaeb1766721d
35 Nb61a0ecbcc1f4b70a9d136d7efdc8e49
36 Nde149ed19c6a4631acf20404cb8f4e9e
37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002106771
38 https://doi.org/10.1007/bf00264859
39 schema:sdDatePublished 2019-04-11T13:49
40 schema:sdLicense https://scigraph.springernature.com/explorer/license/
41 schema:sdPublisher Nb8e2d5958f0e4e10bb5bbb757608b064
42 schema:url http://link.springer.com/10.1007/BF00264859
43 sgo:license sg:explorer/license/
44 sgo:sdDataset articles
45 rdf:type schema:ScholarlyArticle
46 N094e5d87d6b241d2a4d8ab93adc5315b rdf:first sg:person.076111243.63
47 rdf:rest rdf:nil
48 N0b434600db2b4e0cb16fbc13135ce41a schema:name pubmed_id
49 schema:value 4612332
50 rdf:type schema:PropertyValue
51 N10da0508874e4d8999f7052f74948dcc schema:name nlm_unique_id
52 schema:value 0125036
53 rdf:type schema:PropertyValue
54 N3d43fd79c70b4205836ffaeb1766721d schema:name readcube_id
55 schema:value 0a089065e3b2697b2ad748f11ef780a5d7ff37ef1f4799f2802eb7086be240c3
56 rdf:type schema:PropertyValue
57 N5f0373c1a3a541febddd72deea4f2947 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
58 schema:name Saccharomyces cerevisiae
59 rdf:type schema:DefinedTerm
60 N60f625de17444b97bea3c4239076d340 schema:issueNumber 4
61 rdf:type schema:PublicationIssue
62 N68c891f7b0bb4768b68c5625f7d864f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
63 schema:name Ethyl Ethers
64 rdf:type schema:DefinedTerm
65 N78f0c99f5b3946cdad0a1de273a017c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Mutation
67 rdf:type schema:DefinedTerm
68 Na1b4382bc8ec4f3abb5199c9811f4e66 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Spores, Fungal
70 rdf:type schema:DefinedTerm
71 Nb61a0ecbcc1f4b70a9d136d7efdc8e49 schema:name doi
72 schema:value 10.1007/bf00264859
73 rdf:type schema:PropertyValue
74 Nb70b2297c73342dfafbff6e0513d40be rdf:first sg:person.01301763033.64
75 rdf:rest N094e5d87d6b241d2a4d8ab93adc5315b
76 Nb8e2d5958f0e4e10bb5bbb757608b064 schema:name Springer Nature - SN SciGraph project
77 rdf:type schema:Organization
78 Ncf47902c0c45468a827fd49d3843b38a schema:volumeNumber 131
79 rdf:type schema:PublicationVolume
80 Nde149ed19c6a4631acf20404cb8f4e9e schema:name dimensions_id
81 schema:value pub.1002106771
82 rdf:type schema:PropertyValue
83 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
84 schema:name Biological Sciences
85 rdf:type schema:DefinedTerm
86 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
87 schema:name Genetics
88 rdf:type schema:DefinedTerm
89 sg:journal.1297380 schema:issn 1432-1874
90 1617-4615
91 schema:name Molecular Genetics and Genomics
92 rdf:type schema:Periodical
93 sg:person.01301763033.64 schema:affiliation https://www.grid.ac/institutes/grid.4305.2
94 schema:familyName Dawes
95 schema:givenName Ian W.
96 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301763033.64
97 rdf:type schema:Person
98 sg:person.076111243.63 schema:affiliation https://www.grid.ac/institutes/grid.4305.2
99 schema:familyName Hardie
100 schema:givenName Ian D.
101 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.076111243.63
102 rdf:type schema:Person
103 https://app.dimensions.ai/details/publication/pub.1075782231 schema:CreativeWork
104 https://app.dimensions.ai/details/publication/pub.1076162524 schema:CreativeWork
105 https://app.dimensions.ai/details/publication/pub.1076495428 schema:CreativeWork
106 https://app.dimensions.ai/details/publication/pub.1077358086 schema:CreativeWork
107 https://app.dimensions.ai/details/publication/pub.1080455781 schema:CreativeWork
108 https://app.dimensions.ai/details/publication/pub.1080731252 schema:CreativeWork
109 https://app.dimensions.ai/details/publication/pub.1080833544 schema:CreativeWork
110 https://app.dimensions.ai/details/publication/pub.1081018870 schema:CreativeWork
111 https://app.dimensions.ai/details/publication/pub.1081351786 schema:CreativeWork
112 https://doi.org/10.1016/s0065-2911(08)60252-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005151665
113 rdf:type schema:CreativeWork
114 https://doi.org/10.1042/bj1180667 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033290847
115 rdf:type schema:CreativeWork
116 https://doi.org/10.1126/science.158.3802.803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062492637
117 rdf:type schema:CreativeWork
118 https://doi.org/10.1515/znb-1958-1004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002140139
119 rdf:type schema:CreativeWork
120 https://www.grid.ac/institutes/grid.4305.2 schema:alternateName University of Edinburgh
121 schema:name Department of Microbiology, University of Edinburgh, Edinburgh, Scotland
122 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...