Malic acid accumulation by Aspergillus flavus View Full Text


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Article Info

DATE

1989-02

AUTHORS

Yoav Peleg, Ayala Barak, Michael C. Scrutton, Israel Goldberg

ABSTRACT

13C Nuclear magnetic resonance and fumarase and NAD-malate dehydrogenase isoenzyme studies were carried out in a strain of A. flavus which produces relatively high levels of l-malic acid from glucose. The results of the 13C NMR showed that the 13C label from [1-13C] glucose was incorporated only to C-3 (-CH2-) of l-malic acid and indicated that this acid must be synthesized from pyruvate mainly via oxaloacetate. Electrophoretic analysis has established the presence of unique mitochondrial and cytosolic isoenzymes for fumarase and malate dehydrogenase. Changes in the isoenzyme pattern were observed for malate dehydrogenase but not for fumarase during acid production. Cycloheximide inhibited profoundly both l-malic acid production and the increase in the major isoenzyme of malate dehydrogenase, without affecting either the total activity of fumarase or its isoenzyme pattern. The results suggested that de novo protein synthesis is involved in the increase in the activity of the major isoenzyme of malate dehydrogenase and that this isoenzyme is essential for l-malic acid production and accumulation. More... »

PAGES

176-183

References to SciGraph publications

  • 1988-03. Malic acid accumulation by Aspergillus flavus in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1988-03. Malic acid accumulation by Aspergillus flavus in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1007/bf00264008

    DOI

    http://dx.doi.org/10.1007/bf00264008

    DIMENSIONS

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