The regulation of adenylate cyclase in adipocyte plasma membrane from genetically obese (ob/ob) mice View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1984-06

AUTHORS

R. R. French, D. A. York

ABSTRACT

Adenylate cyclase activity was determined using a purified plasma membrane fraction prepared from isolated adipocytes by a Percoll gradient centrifugation procedure. The activation of adenylate cyclase by isoprenaline was reduced by approximately 50% in membranes from obese mice, although no reduction in the sensitivity of the response was apparent. Basal and fluoride- and 5′-guanylyl-imidodiphosphate-stimulated activities were similar in both groups. However, two defects in the obese system were identified which suggested that an alteration in the properties of the regulatory protein may account for the impaired hormonal activation. The specific binding of 3H-dihydroalprenalol to membranes from obese animals also was decreased, suggesting a reduction in the number of β-adrenergic receptors. More... »

PAGES

466-472

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00262223

DOI

http://dx.doi.org/10.1007/bf00262223

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048911765

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6088334


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