Regulation of a modified CaMV 35S promoter by the Tn10-encoded Tet repressor in transgenic tobacco View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-06

AUTHORS

Christiane Gatz, Astrid Kaiser, Regina Wendenburg

ABSTRACT

We have investigated the use of the Tn10-encoded tet repressor-operator system to regulate the expression of a suitably engineered cauliflower mosaic virus (CaMV) 35S promoter in transgenic tobacco plants. First, a transgenic plant was generated which constitutively synthesizes 600000 Tet repressor monomers per cell. In a second transformation step, the β-glucuronidase (gus) gene under the control of a modified CaMV 35S promoter, containing two tet operators, was stably integrated into the plant genome of a tetR+ plant. Expression of the gus gene is repressed 5-fold, if the operators are located flanking the TATA box, and 50- to 80-fold when both operators are positioned downstream of the TATA box. This indicates that Tet repressor-operator complexes can form on plant chromosomes and interfere with transcription. Maximal induction is achieved after 0.5 h upon application of only 0.1 mg/l tetracycline. This fast and efficient induction makes the system useful for specifically inducing expression of transferred genes at different stages of plant development. More... »

PAGES

229-237

Journal

TITLE

Molecular Genetics and Genomics

ISSUE

2

VOLUME

227

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00259675

    DOI

    http://dx.doi.org/10.1007/bf00259675

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1027834012

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2062303


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