Production of somatic hybrids by electrofusion in Solanum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-08

AUTHORS

N. Fish, A. Karp, M. G. K. Jones

ABSTRACT

Conditions are described for large scale electrofusion of mesophyll protoplasts of dihaploid S. tuberosum with those of diploid S. brevidens. Overall fusion frequencies of 20%-30% were achieved, and following fusion, large numbers of protoplast-derived calli were obtained. Putative somatic hybrid plants were selected from the regenerated shoots by examining their morphological characteristics. Twenty-one somatic hybrids were confirmed by isoenzyme analysis and six somatic hybrids were further confirmed by Southern hybridization. Tetraploid hybrids were obtained, but cytogenetic studies indicated that more of the regenerated hybrids were hexaploid than had previously been found following chemical fusion of the same partners. Some advantages of electrofusion over chemical fusion are discussed. More... »

PAGES

260-266

References to SciGraph publications

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  • 1983-10. Improved culture ability of potato protoplasts by use of activated charcoal in PLANT CELL REPORTS
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  • 1985-12. Intra-specific fusions in Solanum tuberosum in THEORETICAL AND APPLIED GENETICS
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  • 1983-07. Complementation analysis of nitrate reductase deficient mutants of Nicotiana tabacum by somatic hybridization in THEORETICAL AND APPLIED GENETICS
  • 1982-09. Chromosome variation in protoplast-derived potato plants in THEORETICAL AND APPLIED GENETICS
  • 1986-10. The effect of chemical facilitators on the frequency of electrofusion of tobacco mesophyll protoplast in PLANT CELL REPORTS
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  • 1985-06. Culture of plant somatic hybrids following electrical fusion in THEORETICAL AND APPLIED GENETICS
  • 1984-04. Species-specific repetitive DNA used to identify interspecific somatic hybrids in PLANT CELL REPORTS
  • 1979-03. Comparison of single cell culture derived Solanum tuberosum L. plants and a model for their application in breeding programs in THEORETICAL AND APPLIED GENETICS
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  • Journal

    TITLE

    Theoretical and Applied Genetics

    ISSUE

    2

    VOLUME

    76

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00257854

    DOI

    http://dx.doi.org/10.1007/bf00257854

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1006138863

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24232114


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