A selection method for the synthesis of triploid hybrids by fusion of microspore protoplasts (n) with somatic cell protoplasts (2n) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-08

AUTHORS

D. Pental, A. Mukhopadhyay, A. Grover, A. K. Pradhan

ABSTRACT

Microspore protoplasts (n) isolated at the tetrad stage from plants of Nicotiana tabacum Km(+) (2n=4x=48) were fused with somatic cell protoplasts (2n) of WT N. rustica (2n=4x=48) to produce triploid plants. A total of 21.2×10(6) microspore protoplasts were fused with 11.2×10(6) somatic cell protoplasts using the high pH/Ca(+ +) method. Microspore protoplasts did not divide and WT N. rustica protoplasts stopped dividing when the protoplast-derived colonies were transferred to a selection medium containing kanamycin. A total of 104 actively growing green colonies were recovered on the selection medium. Ninety-six of these colonies were tested for their hybrid nature by PAGE of peroxidases and were found to contain bands characteristic of both parents. Hybrid nature of the plants regenerated from some of the selected colonies was confirmed by IEF of leaf esterases, by NPT II activity assay and by hybridizing total DNAs restricted with EcoR I to a cloned 18s rDNA fragment. Root tip squashes of six of the hybrid plants revealed chromosome numbers ranging from 58-72. From chromosomal and biochemical analysis, it can be concluded that the procedure of fusing microspore protoplasts (n) of species A carrying a dominant selection marker with WT somatic cell protoplasts (2n) of species B can be a convenient selection method for the synthesis of triploid plants. The significance of triploids lies in their subsequent use for transferring alien chromosomes and genes of species A to species B. More... »

PAGES

237-243

References to SciGraph publications

  • 1987-03. Selection of a universal hybridizer in Sinapis turgida Del. and regeneration of plantlets from somatic hybrids with Brassica species in PLANTA
  • 1985-08. Electrical fusion for optimal formation of protoplast heterokaryons in Nicotiana in PLANTA
  • 1987-10. Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts in THEORETICAL AND APPLIED GENETICS
  • 1986-04. The production of fertile, triploid somatic hybrid plants (Nicotiana glutinosa (n) + N. tabacum (2n)) via gametic: somatic protoplast fusion in THEORETICAL AND APPLIED GENETICS
  • 1974-12. Somatic hybridisation of plants by fusion of protoplasts in MOLECULAR GENETICS AND GENOMICS
  • 1987-04. High efficiency Agrobacterium-mediated transformation of Lycopersicon based on conditions favorable for regeneration in PLANT CELL REPORTS
  • 1984-08. Somatic hybridisation using a double mutant of nicotiana tabacum in HEREDITY
  • 1982-12. Correction of nitrate reductase defect in auxotrophic plant cells through protoplast-mediated intergeneric gene transfers in MOLECULAR GENETICS AND GENOMICS
  • 1986-04. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens in PLANT CELL REPORTS
  • 1987-06. Interspecific T-DNA transfer through plant protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • 1984-10. Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • 1986-03. Somatic hybridization of Nicotiana tabacum and Petunia hybrida in MOLECULAR GENETICS AND GENOMICS
  • 1972-09. Isolation of Protoplasts from Pollen Tetrads in NATURE
  • 1987-07. Somatic hybridization in potato: use of γ-irradiated protoplasts of Solanum pinnatisectum in genetic reconstruction in THEORETICAL AND APPLIED GENETICS
  • 1985-05. Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts in MOLECULAR GENETICS AND GENOMICS
  • 1987-08. Transgenic Plants of Brassica napus L. in NATURE BIOTECHNOLOGY
  • 1987-03. Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants in NATURE BIOTECHNOLOGY
  • 1987-07. Selection of a Nicotiana plumbaginifolia universal hybridizer and its use in intergeneric somatic hybrid formation in MOLECULAR GENETICS AND GENOMICS
  • 1984-04. A simple method for large-scale electrofusion and culture of plant protoplasts in BIOSCIENCE REPORTS
  • 1987-07. Combination of kanamycin resistance and nitrate reductase deficiency as selectable markers in one nuclear genome provides a universal somatic hybridizer in plants in MOLECULAR GENETICS AND GENOMICS
  • 1985-12. Analysis of fertility in somatic hybrids of Nicotiana rustica and N. tabacum and progeny over two sexual generations in THEORETICAL AND APPLIED GENETICS
  • 1981-01. High frequency fusion of plant protoplasts by electric fields in PLANTA
  • 1984-09. Novel alloplasmic Nicotiana plants by “donor-recipient” protoplast fusion: cybrids having N. tabacum or N. sylvestris nuclear genomes and either or both plastomes and chondriomes from alien species in MOLECULAR GENETICS AND GENOMICS
  • 1985-12. Some theoretical and practical possibilities of plant genetic manipulation using protoplasts in HEREDITAS
  • 1987-08. X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products in THEORETICAL AND APPLIED GENETICS
  • 1980-09. Intergeneric gene transfer mediated by plant protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • 1987-10. Transformation of Brassica napus with Agrobacterium tumefaciens based vectors in PLANT CELL REPORTS
  • 1987-10. Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase in PLANT CELL REPORTS
  • 1986-04. Transformation of Medicago by Agrobacterium mediated gene transfer in PLANT CELL REPORTS
  • 1987-02. Agrobacterium mediated transformation and regeneration of Populus in MOLECULAR GENETICS AND GENOMICS
  • 1986-09. Transformation of cultivated tomato by a binary vector in Agrobacterium rhizogenes: transgenic plants with normal phenotypes harbor binary vector T-DNA, but no Ri-plasmid T-DNA in THEORETICAL AND APPLIED GENETICS
  • 1983-03. Transfer of cytoplasmic male sterility by selection for streptomycin resistance after protoplast fusion in Nicotiana in MOLECULAR GENETICS AND GENOMICS
  • 1972-09. Excitable Membranes in NATURE
  • 1987-11. Genetic Engineering of Sunflower (Helianthus Annuus L.) in NATURE BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00257851

    DOI

    http://dx.doi.org/10.1007/bf00257851

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1023839362

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24232111


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