Sodium and potassium currents recorded during an action potential View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-04-01

AUTHORS

V. de Hass, W. Vogel

ABSTRACT

A simple method was used to measure directly sodium and potassium currents underlying the action potential in single nerve fibres of Xenopus laevis. A short rectangular stimulus under current-clamp conditions elicited an action potential which was digitally stored and later used as command when voltageclamping the same fibre. The currents thus obtained nearly reproduced the original rectangular stimulus. Adding first 100 nM TTX and subsequently 100 nM TTX plus 10 mM TEA to the extracellular Ringer solution revealed the sodium and the potassium currents during an action potential. They were converted to permeabilities by use of the constant-field equation and are in good agreement with the curves which had been calculated from conventional voltage-clamp data. Thus experimentally determined currents and permeabilities are shown as they are changing during an action potential. More... »

PAGES

49-51

References to SciGraph publications

  • 1969-06. A new voltage clamp method for Ranvier nodes in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00257145

    DOI

    http://dx.doi.org/10.1007/bf00257145

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1001902228

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2546753


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/02", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Physical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0202", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Atomic, Molecular, Nuclear, Particle and Plasma Physics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0299", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Other Physical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Action Potentials", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Electric Stimulation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "In Vitro Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nerve Fibers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Potassium Channels", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sciatic Nerve", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sodium Channels", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tetrodotoxin", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Xenopus laevis", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Physiologisches Institut der Justus-Liebig-Universit\u00e4t Giessen, Aulweg 129, D-6300, Giessen, Federal Republic of Germany", 
              "id": "http://www.grid.ac/institutes/grid.8664.c", 
              "name": [
                "Physiologisches Institut der Justus-Liebig-Universit\u00e4t Giessen, Aulweg 129, D-6300, Giessen, Federal Republic of Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "de Hass", 
            "givenName": "V.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Physiologisches Institut der Justus-Liebig-Universit\u00e4t Giessen, Aulweg 129, D-6300, Giessen, Federal Republic of Germany", 
              "id": "http://www.grid.ac/institutes/grid.8664.c", 
              "name": [
                "Physiologisches Institut der Justus-Liebig-Universit\u00e4t Giessen, Aulweg 129, D-6300, Giessen, Federal Republic of Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Vogel", 
            "givenName": "W.", 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00586967", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012003057", 
              "https://doi.org/10.1007/bf00586967"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1989-04-01", 
        "datePublishedReg": "1989-04-01", 
        "description": "A simple method was used to measure directly sodium and potassium currents underlying the action potential in single nerve fibres of Xenopus laevis. A short rectangular stimulus under current-clamp conditions elicited an action potential which was digitally stored and later used as command when voltageclamping the same fibre. The currents thus obtained nearly reproduced the original rectangular stimulus. Adding first 100 nM TTX and subsequently 100 nM TTX plus 10 mM TEA to the extracellular Ringer solution revealed the sodium and the potassium currents during an action potential. They were converted to permeabilities by use of the constant-field equation and are in good agreement with the curves which had been calculated from conventional voltage-clamp data. Thus experimentally determined currents and permeabilities are shown as they are changing during an action potential.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/bf00257145", 
        "inLanguage": "en", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1313667", 
            "issn": [
              "0175-7571", 
              "1432-1017"
            ], 
            "name": "European Biophysics Journal", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "17"
          }
        ], 
        "keywords": [
          "action potentials", 
          "potassium current", 
          "nM TTX", 
          "rectangular stimuli", 
          "current-clamp conditions", 
          "single nerve fibers", 
          "nerve fibers", 
          "Ringer's solution", 
          "TTX", 
          "voltage-clamp data", 
          "sodium", 
          "stimuli", 
          "Xenopus laevis", 
          "constant field equation", 
          "same fiber", 
          "tea", 
          "potential", 
          "fibers", 
          "permeability", 
          "laevis", 
          "use", 
          "data", 
          "curves", 
          "simple method", 
          "conditions", 
          "method", 
          "current", 
          "command", 
          "agreement", 
          "solution", 
          "good agreement", 
          "equations"
        ], 
        "name": "Sodium and potassium currents recorded during an action potential", 
        "pagination": "49-51", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1001902228"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00257145"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "2546753"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00257145", 
          "https://app.dimensions.ai/details/publication/pub.1001902228"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-06-01T21:59", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_189.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/bf00257145"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00257145'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00257145'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00257145'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00257145'


     

    This table displays all metadata directly associated to this object as RDF triples.

    155 TRIPLES      22 PREDICATES      72 URIs      60 LITERALS      17 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00257145 schema:about N31a2fa0a28844dee80cc727a741dc47b
    2 N3e2f69037bd044d0a51f5461a01028cd
    3 N5a9e93cd6d7342f9b16e322a20877cf9
    4 N5ad3a393c93f445c8bbf3579887b0d98
    5 N61ab88ca08cc4e8d9b9920a243304c59
    6 N635341edd1a24f9eaba26a8f8f6b2635
    7 N7f2bfa3f3cb14a2ea6dc010db6c941dc
    8 Na445218b582d4b1a97dca18784ffb15f
    9 Ndfdaa524798846d788e65593cd1a36d5
    10 Nfdd060c960f8484d8edb10a5e4ea3eb7
    11 anzsrc-for:02
    12 anzsrc-for:0202
    13 anzsrc-for:0299
    14 anzsrc-for:06
    15 anzsrc-for:0601
    16 schema:author Nf2931a1850144d0ca492d40ae1afbf07
    17 schema:citation sg:pub.10.1007/bf00586967
    18 schema:datePublished 1989-04-01
    19 schema:datePublishedReg 1989-04-01
    20 schema:description A simple method was used to measure directly sodium and potassium currents underlying the action potential in single nerve fibres of Xenopus laevis. A short rectangular stimulus under current-clamp conditions elicited an action potential which was digitally stored and later used as command when voltageclamping the same fibre. The currents thus obtained nearly reproduced the original rectangular stimulus. Adding first 100 nM TTX and subsequently 100 nM TTX plus 10 mM TEA to the extracellular Ringer solution revealed the sodium and the potassium currents during an action potential. They were converted to permeabilities by use of the constant-field equation and are in good agreement with the curves which had been calculated from conventional voltage-clamp data. Thus experimentally determined currents and permeabilities are shown as they are changing during an action potential.
    21 schema:genre article
    22 schema:inLanguage en
    23 schema:isAccessibleForFree false
    24 schema:isPartOf N6e4a3add39434f7cb4a410181a1eacce
    25 N880608e37d1b4615ade0cd700fd6de47
    26 sg:journal.1313667
    27 schema:keywords Ringer's solution
    28 TTX
    29 Xenopus laevis
    30 action potentials
    31 agreement
    32 command
    33 conditions
    34 constant field equation
    35 current
    36 current-clamp conditions
    37 curves
    38 data
    39 equations
    40 fibers
    41 good agreement
    42 laevis
    43 method
    44 nM TTX
    45 nerve fibers
    46 permeability
    47 potassium current
    48 potential
    49 rectangular stimuli
    50 same fiber
    51 simple method
    52 single nerve fibers
    53 sodium
    54 solution
    55 stimuli
    56 tea
    57 use
    58 voltage-clamp data
    59 schema:name Sodium and potassium currents recorded during an action potential
    60 schema:pagination 49-51
    61 schema:productId N058d809b16294be28e28de503e60ec93
    62 Nafd6cfd709d54f4ea8f0fe27d829a87a
    63 Ncaa6df540dc049c39b2d47eab19e063f
    64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001902228
    65 https://doi.org/10.1007/bf00257145
    66 schema:sdDatePublished 2022-06-01T21:59
    67 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    68 schema:sdPublisher N87d338d95b4d4fda8935dd084d619e0f
    69 schema:url https://doi.org/10.1007/bf00257145
    70 sgo:license sg:explorer/license/
    71 sgo:sdDataset articles
    72 rdf:type schema:ScholarlyArticle
    73 N058d809b16294be28e28de503e60ec93 schema:name doi
    74 schema:value 10.1007/bf00257145
    75 rdf:type schema:PropertyValue
    76 N31a2fa0a28844dee80cc727a741dc47b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    77 schema:name Action Potentials
    78 rdf:type schema:DefinedTerm
    79 N3deed4e87dd84fd5a0a5a3aeb3d581a2 schema:affiliation grid-institutes:grid.8664.c
    80 schema:familyName Vogel
    81 schema:givenName W.
    82 rdf:type schema:Person
    83 N3e2f69037bd044d0a51f5461a01028cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    84 schema:name Tetrodotoxin
    85 rdf:type schema:DefinedTerm
    86 N5a9e93cd6d7342f9b16e322a20877cf9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    87 schema:name Electric Stimulation
    88 rdf:type schema:DefinedTerm
    89 N5ad3a393c93f445c8bbf3579887b0d98 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Xenopus laevis
    91 rdf:type schema:DefinedTerm
    92 N61ab88ca08cc4e8d9b9920a243304c59 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name In Vitro Techniques
    94 rdf:type schema:DefinedTerm
    95 N635341edd1a24f9eaba26a8f8f6b2635 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Sciatic Nerve
    97 rdf:type schema:DefinedTerm
    98 N6e4a3add39434f7cb4a410181a1eacce schema:issueNumber 1
    99 rdf:type schema:PublicationIssue
    100 N7f2bfa3f3cb14a2ea6dc010db6c941dc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    101 schema:name Sodium Channels
    102 rdf:type schema:DefinedTerm
    103 N87d338d95b4d4fda8935dd084d619e0f schema:name Springer Nature - SN SciGraph project
    104 rdf:type schema:Organization
    105 N880608e37d1b4615ade0cd700fd6de47 schema:volumeNumber 17
    106 rdf:type schema:PublicationVolume
    107 Na445218b582d4b1a97dca18784ffb15f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Potassium Channels
    109 rdf:type schema:DefinedTerm
    110 Nafd6cfd709d54f4ea8f0fe27d829a87a schema:name dimensions_id
    111 schema:value pub.1001902228
    112 rdf:type schema:PropertyValue
    113 Nc8b6034792364598b404f0c0b132c69f rdf:first N3deed4e87dd84fd5a0a5a3aeb3d581a2
    114 rdf:rest rdf:nil
    115 Ncaa6df540dc049c39b2d47eab19e063f schema:name pubmed_id
    116 schema:value 2546753
    117 rdf:type schema:PropertyValue
    118 Nd7a60e096c19475dae1308ba4fbb11aa schema:affiliation grid-institutes:grid.8664.c
    119 schema:familyName de Hass
    120 schema:givenName V.
    121 rdf:type schema:Person
    122 Ndfdaa524798846d788e65593cd1a36d5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Animals
    124 rdf:type schema:DefinedTerm
    125 Nf2931a1850144d0ca492d40ae1afbf07 rdf:first Nd7a60e096c19475dae1308ba4fbb11aa
    126 rdf:rest Nc8b6034792364598b404f0c0b132c69f
    127 Nfdd060c960f8484d8edb10a5e4ea3eb7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Nerve Fibers
    129 rdf:type schema:DefinedTerm
    130 anzsrc-for:02 schema:inDefinedTermSet anzsrc-for:
    131 schema:name Physical Sciences
    132 rdf:type schema:DefinedTerm
    133 anzsrc-for:0202 schema:inDefinedTermSet anzsrc-for:
    134 schema:name Atomic, Molecular, Nuclear, Particle and Plasma Physics
    135 rdf:type schema:DefinedTerm
    136 anzsrc-for:0299 schema:inDefinedTermSet anzsrc-for:
    137 schema:name Other Physical Sciences
    138 rdf:type schema:DefinedTerm
    139 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    140 schema:name Biological Sciences
    141 rdf:type schema:DefinedTerm
    142 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    143 schema:name Biochemistry and Cell Biology
    144 rdf:type schema:DefinedTerm
    145 sg:journal.1313667 schema:issn 0175-7571
    146 1432-1017
    147 schema:name European Biophysics Journal
    148 schema:publisher Springer Nature
    149 rdf:type schema:Periodical
    150 sg:pub.10.1007/bf00586967 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012003057
    151 https://doi.org/10.1007/bf00586967
    152 rdf:type schema:CreativeWork
    153 grid-institutes:grid.8664.c schema:alternateName Physiologisches Institut der Justus-Liebig-Universität Giessen, Aulweg 129, D-6300, Giessen, Federal Republic of Germany
    154 schema:name Physiologisches Institut der Justus-Liebig-Universität Giessen, Aulweg 129, D-6300, Giessen, Federal Republic of Germany
    155 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...