Production of human monoclonal antibodies by heterohybridomas View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-07

AUTHORS

Kazuaki Kitano, Yasushi Shintani, Yuzo Ichimori, Kyozo Tsukamoto, Seijiro Sasai, Makoto Kida

ABSTRACT

Mouse human-human heterohybridomas secreting human monoclonal antibodies (MoAb) against tetanus toxoid and hepatitis B virus surface antigen were effectively cultivated in a medium containing a serum substitute called GFS, a 55% to 70% ammonium sulphate fraction of serum from adult cattle. A perfusion culture system using a jar fermentor equipped with a cell sedimentation column with a double jacket was developed and applied to produce human MoAb. In this fermentor, maximum cell density of a heterohybridoma reached 1.2×107 cells/ml and MoAb was continuously accumulated at a constant rate for at least 40 days; this led to the production of more than one gram of human MoAb using a culture vessel with a 1-1 working volume. More... »

PAGES

282-286

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00257050

DOI

http://dx.doi.org/10.1007/bf00257050

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039787667


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