Ontology type: schema:ScholarlyArticle
1986-07
AUTHORSKazuaki Kitano, Yasushi Shintani, Yuzo Ichimori, Kyozo Tsukamoto, Seijiro Sasai, Makoto Kida
ABSTRACTMouse human-human heterohybridomas secreting human monoclonal antibodies (MoAb) against tetanus toxoid and hepatitis B virus surface antigen were effectively cultivated in a medium containing a serum substitute called GFS, a 55% to 70% ammonium sulphate fraction of serum from adult cattle. A perfusion culture system using a jar fermentor equipped with a cell sedimentation column with a double jacket was developed and applied to produce human MoAb. In this fermentor, maximum cell density of a heterohybridoma reached 1.2×107 cells/ml and MoAb was continuously accumulated at a constant rate for at least 40 days; this led to the production of more than one gram of human MoAb using a culture vessel with a 1-1 working volume. More... »
PAGES282-286
http://scigraph.springernature.com/pub.10.1007/bf00257050
DOIhttp://dx.doi.org/10.1007/bf00257050
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1039787667
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Immunology",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Central Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan",
"id": "http://www.grid.ac/institutes/grid.419841.1",
"name": [
"Central Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan"
],
"type": "Organization"
},
"familyName": "Kitano",
"givenName": "Kazuaki",
"id": "sg:person.010714335450.85",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010714335450.85"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Central Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan",
"id": "http://www.grid.ac/institutes/grid.419841.1",
"name": [
"Central Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan"
],
"type": "Organization"
},
"familyName": "Shintani",
"givenName": "Yasushi",
"id": "sg:person.01131236461.56",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131236461.56"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Central Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan",
"id": "http://www.grid.ac/institutes/grid.419841.1",
"name": [
"Central Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan"
],
"type": "Organization"
},
"familyName": "Ichimori",
"givenName": "Yuzo",
"id": "sg:person.01322123072.36",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322123072.36"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Central Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan",
"id": "http://www.grid.ac/institutes/grid.419841.1",
"name": [
"Central Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan"
],
"type": "Organization"
},
"familyName": "Tsukamoto",
"givenName": "Kyozo",
"id": "sg:person.01243167027.95",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243167027.95"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Hikari Plant, Takeda Chemical Industries Ltd., 743, Hikari, Yamaguchi, Japan",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Hikari Plant, Takeda Chemical Industries Ltd., 743, Hikari, Yamaguchi, Japan"
],
"type": "Organization"
},
"familyName": "Sasai",
"givenName": "Seijiro",
"id": "sg:person.01151256272.21",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151256272.21"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Central Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan",
"id": "http://www.grid.ac/institutes/grid.419841.1",
"name": [
"Central Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan"
],
"type": "Organization"
},
"familyName": "Kida",
"givenName": "Makoto",
"id": "sg:person.012673066145.03",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012673066145.03"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/scientificamerican0183-36",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056482673",
"https://doi.org/10.1038/scientificamerican0183-36"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/242165a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038610548",
"https://doi.org/10.1038/242165a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/269420a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001707449",
"https://doi.org/10.1038/269420a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/256495a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001387921",
"https://doi.org/10.1038/256495a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02618284",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052909169",
"https://doi.org/10.1007/bf02618284"
],
"type": "CreativeWork"
}
],
"datePublished": "1986-07",
"datePublishedReg": "1986-07-01",
"description": "Mouse human-human heterohybridomas secreting human monoclonal antibodies (MoAb) against tetanus toxoid and hepatitis B virus surface antigen were effectively cultivated in a medium containing a serum substitute called GFS, a 55% to 70% ammonium sulphate fraction of serum from adult cattle. A perfusion culture system using a jar fermentor equipped with a cell sedimentation column with a double jacket was developed and applied to produce human MoAb. In this fermentor, maximum cell density of a heterohybridoma reached 1.2\u00d7107 cells/ml and MoAb was continuously accumulated at a constant rate for at least 40 days; this led to the production of more than one gram of human MoAb using a culture vessel with a 1-1 working volume.",
"genre": "article",
"id": "sg:pub.10.1007/bf00257050",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1083533",
"issn": [
"0175-7598",
"1432-0614"
],
"name": "Applied Microbiology and Biotechnology",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "4",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "24"
}
],
"keywords": [
"human monoclonal antibody",
"human MoAbs",
"monoclonal antibodies",
"hepatitis B virus surface antigen",
"virus surface antigen",
"tetanus toxoid",
"surface antigen",
"MoAb",
"cells/",
"adult cattle",
"heterohybridomas",
"antibodies",
"serum substitute",
"culture system",
"cell density",
"toxoid",
"sulfate fraction",
"antigen",
"serum",
"ammonium sulfate fraction",
"days",
"perfusion culture system",
"vessels",
"rate",
"production",
"volume",
"constant rate",
"cattle",
"substitute",
"fraction",
"culture vessels",
"medium",
"GFS",
"maximum cell density",
"system",
"density",
"column",
"double jacket",
"jacket",
"jar fermentor",
"fermentor",
"working volume",
"sedimentation column"
],
"name": "Production of human monoclonal antibodies by heterohybridomas",
"pagination": "282-286",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1039787667"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00257050"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00257050",
"https://app.dimensions.ai/details/publication/pub.1039787667"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:18",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_213.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/bf00257050"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00257050'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00257050'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00257050'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00257050'
This table displays all metadata directly associated to this object as RDF triples.
159 TRIPLES
22 PREDICATES
74 URIs
61 LITERALS
6 BLANK NODES