Transport of lactate and other short-chain monocarboxylates in the yeast Candida utilis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-02

AUTHORS

Cecilia Leão, N. van Uden

ABSTRACT

Lactic acid grown cells of the yeast Candida utilis transported lactate by an accumulative electroneutral proton-lactate symport with a proton-lactate stoicheiometry of 1:1. The accumulation ratio at pH 5.5 was about twenty. The symport accepted the following monocarboxylates (Ksvalues at 25°C, pH 5.5 in brackets): d-lactate (0.06 mM), l-lactate (0.06 mM), pyruvate (0.03 mM), propionate (0.05 mM) and acetate (0.1 mM). The system was inducible and was subject to glucose repression. The affinity of the symport for lactate was not affected by pH over the range 3–6, while the maximum transport velocity was strongly pH dependent, its optimum pH being around pH 5. Undissociated lactic acid entered the cells by simple diffusion. The permeability for the undissociated acid increased exponentially with pH, the diffusion constant increasing 35-fold when the pH was increased from 3 to 5.5. More... »

PAGES

389-393

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00257039

DOI

http://dx.doi.org/10.1007/bf00257039

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005925377


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Minho", 
          "id": "https://www.grid.ac/institutes/grid.10328.38", 
          "name": [
            "Laboratory of Biology, Univesity of Minho, Braga, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Le\u00e3o", 
        "givenName": "Cecilia", 
        "id": "sg:person.01127702535.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127702535.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Instituto Gulbenkian de Ci\u00eancia", 
          "id": "https://www.grid.ac/institutes/grid.418346.c", 
          "name": [
            "Laboratory of Microbiology, Gulbenkian Institute of Science, 2781, Oeiras Codex, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van Uden", 
        "givenName": "N.", 
        "id": "sg:person.07571423510.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07571423510.66"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0005-2736(84)90272-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005981119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(84)90272-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005981119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(83)90232-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011395048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(83)90232-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011395048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1720015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043816543"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1720015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043816543"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4157(81)90002-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044209037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4157(81)90002-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044209037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00406676", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050831322", 
          "https://doi.org/10.1007/bf00406676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00406676", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050831322", 
          "https://doi.org/10.1007/bf00406676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077155216", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1986-02", 
    "datePublishedReg": "1986-02-01", 
    "description": "Lactic acid grown cells of the yeast Candida utilis transported lactate by an accumulative electroneutral proton-lactate symport with a proton-lactate stoicheiometry of 1:1. The accumulation ratio at pH 5.5 was about twenty. The symport accepted the following monocarboxylates (Ksvalues at 25\u00b0C, pH 5.5 in brackets): d-lactate (0.06 mM), l-lactate (0.06 mM), pyruvate (0.03 mM), propionate (0.05 mM) and acetate (0.1 mM). The system was inducible and was subject to glucose repression. The affinity of the symport for lactate was not affected by pH over the range 3\u20136, while the maximum transport velocity was strongly pH dependent, its optimum pH being around pH 5. Undissociated lactic acid entered the cells by simple diffusion. The permeability for the undissociated acid increased exponentially with pH, the diffusion constant increasing 35-fold when the pH was increased from 3 to 5.5.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00257039", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "23"
      }
    ], 
    "name": "Transport of lactate and other short-chain monocarboxylates in the yeast Candida utilis", 
    "pagination": "389-393", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "1c3a3b51774fe6b3a463a35067975942655bf42645edc075dc5dca8c193dd83c"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00257039"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1005925377"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00257039", 
      "https://app.dimensions.ai/details/publication/pub.1005925377"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130826_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00257039"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00257039'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00257039'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00257039'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00257039'


 

This table displays all metadata directly associated to this object as RDF triples.

89 TRIPLES      21 PREDICATES      33 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00257039 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N27292b4da3ed4186a3ab1e0feeee359c
4 schema:citation sg:pub.10.1007/bf00406676
5 https://app.dimensions.ai/details/publication/pub.1077155216
6 https://doi.org/10.1016/0005-2736(83)90232-8
7 https://doi.org/10.1016/0005-2736(84)90272-4
8 https://doi.org/10.1016/0304-4157(81)90002-2
9 https://doi.org/10.1042/bj1720015
10 schema:datePublished 1986-02
11 schema:datePublishedReg 1986-02-01
12 schema:description Lactic acid grown cells of the yeast Candida utilis transported lactate by an accumulative electroneutral proton-lactate symport with a proton-lactate stoicheiometry of 1:1. The accumulation ratio at pH 5.5 was about twenty. The symport accepted the following monocarboxylates (Ksvalues at 25°C, pH 5.5 in brackets): d-lactate (0.06 mM), l-lactate (0.06 mM), pyruvate (0.03 mM), propionate (0.05 mM) and acetate (0.1 mM). The system was inducible and was subject to glucose repression. The affinity of the symport for lactate was not affected by pH over the range 3–6, while the maximum transport velocity was strongly pH dependent, its optimum pH being around pH 5. Undissociated lactic acid entered the cells by simple diffusion. The permeability for the undissociated acid increased exponentially with pH, the diffusion constant increasing 35-fold when the pH was increased from 3 to 5.5.
13 schema:genre research_article
14 schema:inLanguage en
15 schema:isAccessibleForFree false
16 schema:isPartOf Na9fed1cc3bd548bd811258a23ca64308
17 Nacce1f28cb194b45994546710e6246d3
18 sg:journal.1083533
19 schema:name Transport of lactate and other short-chain monocarboxylates in the yeast Candida utilis
20 schema:pagination 389-393
21 schema:productId N17059293287c409facb160447f614bce
22 N571ceafb114e45968983d5c9d9698275
23 Nede800e34822440e9edc8933b0eeacf6
24 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005925377
25 https://doi.org/10.1007/bf00257039
26 schema:sdDatePublished 2019-04-11T13:59
27 schema:sdLicense https://scigraph.springernature.com/explorer/license/
28 schema:sdPublisher N50ca41ed478c4b28ac29148c27567e63
29 schema:url http://link.springer.com/10.1007/BF00257039
30 sgo:license sg:explorer/license/
31 sgo:sdDataset articles
32 rdf:type schema:ScholarlyArticle
33 N17059293287c409facb160447f614bce schema:name readcube_id
34 schema:value 1c3a3b51774fe6b3a463a35067975942655bf42645edc075dc5dca8c193dd83c
35 rdf:type schema:PropertyValue
36 N27292b4da3ed4186a3ab1e0feeee359c rdf:first sg:person.01127702535.04
37 rdf:rest N69adb113915c4ac587f99481273f60da
38 N50ca41ed478c4b28ac29148c27567e63 schema:name Springer Nature - SN SciGraph project
39 rdf:type schema:Organization
40 N571ceafb114e45968983d5c9d9698275 schema:name dimensions_id
41 schema:value pub.1005925377
42 rdf:type schema:PropertyValue
43 N69adb113915c4ac587f99481273f60da rdf:first sg:person.07571423510.66
44 rdf:rest rdf:nil
45 Na9fed1cc3bd548bd811258a23ca64308 schema:issueNumber 5
46 rdf:type schema:PublicationIssue
47 Nacce1f28cb194b45994546710e6246d3 schema:volumeNumber 23
48 rdf:type schema:PublicationVolume
49 Nede800e34822440e9edc8933b0eeacf6 schema:name doi
50 schema:value 10.1007/bf00257039
51 rdf:type schema:PropertyValue
52 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
53 schema:name Biological Sciences
54 rdf:type schema:DefinedTerm
55 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
56 schema:name Biochemistry and Cell Biology
57 rdf:type schema:DefinedTerm
58 sg:journal.1083533 schema:issn 0175-7598
59 1432-0614
60 schema:name Applied Microbiology and Biotechnology
61 rdf:type schema:Periodical
62 sg:person.01127702535.04 schema:affiliation https://www.grid.ac/institutes/grid.10328.38
63 schema:familyName Leão
64 schema:givenName Cecilia
65 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127702535.04
66 rdf:type schema:Person
67 sg:person.07571423510.66 schema:affiliation https://www.grid.ac/institutes/grid.418346.c
68 schema:familyName van Uden
69 schema:givenName N.
70 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07571423510.66
71 rdf:type schema:Person
72 sg:pub.10.1007/bf00406676 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050831322
73 https://doi.org/10.1007/bf00406676
74 rdf:type schema:CreativeWork
75 https://app.dimensions.ai/details/publication/pub.1077155216 schema:CreativeWork
76 https://doi.org/10.1016/0005-2736(83)90232-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011395048
77 rdf:type schema:CreativeWork
78 https://doi.org/10.1016/0005-2736(84)90272-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005981119
79 rdf:type schema:CreativeWork
80 https://doi.org/10.1016/0304-4157(81)90002-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044209037
81 rdf:type schema:CreativeWork
82 https://doi.org/10.1042/bj1720015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043816543
83 rdf:type schema:CreativeWork
84 https://www.grid.ac/institutes/grid.10328.38 schema:alternateName University of Minho
85 schema:name Laboratory of Biology, Univesity of Minho, Braga, Portugal
86 rdf:type schema:Organization
87 https://www.grid.ac/institutes/grid.418346.c schema:alternateName Instituto Gulbenkian de Ciência
88 schema:name Laboratory of Microbiology, Gulbenkian Institute of Science, 2781, Oeiras Codex, Portugal
89 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...