Malic acid accumulation by Aspergillus flavus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-03

AUTHORS

Yoav Peleg, Barry Stieglitz, Israel Goldberg

ABSTRACT

The accumulation and excretion of l-malic acid, and to a lesser extent succinic and fumaric acids, by Aspergillus flavus occurs under aerobic conditions in a medium containing a high glucose concentration, a limiting amount of nitrogen and a neutralizing agent (CaCO3). An overall malic, succinic, and fumaric acid molar yield of up to 68.6% (moles of acid produced per mole of glucose utilized) is obtained after incubation for 6 to 8 days, although a transient molar yield of 76% was measured. Glucose consumption and formation of acids were faster in a fermentor than in shake flasks. During the acid production stage, the activity of malate dehydrogenase increased 6 to 10-fold while that of fumarase changed only slightly. Cycloheximide greatly inhibited both l-malic acid production and the increase in malate dehydrogenase activity, without affecting fumarase activity. The results suggest that de novo protein synthesis is involved in the increase of malate dehydrogenase and that this enzyme is essential for l-malic acid production and accumulation. More... »

PAGES

69-75

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00250501

DOI

http://dx.doi.org/10.1007/bf00250501

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037109146


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146 rdf:type schema:Organization
147 https://www.grid.ac/institutes/grid.9619.7 schema:alternateName Hebrew University of Jerusalem
148 schema:name Department of Applied Microbiology, Institute of Microbiology, The Hebrew University-Hadassah Medical School, P.O. Box 1172, 91010, Jerusalem, Israel
149 rdf:type schema:Organization
 




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