Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-10

AUTHORS

G. W. Bates, C. A. Hasenkampf, C. L. Contolini, W. C. Piastuch

ABSTRACT

Mesophyll protoplasts of a kanamycin-resistant, nopaline-positive Nicotiana plumbaginifolia seed line were inactivated by γ-irradiation and electrically fused with unirradiated mesophyll protoplasts of N. tabacum. Hybrids were selected on kanamycin and regenerated. Genetic material from N. plumbaginifolia was detected in these plants by the following criteria: (1) morphology, (2) esterase isozyme profiles, and (3) the presence of nopaline in leaf extracts. All of the plants regenerated were morphologically more similar to N. tabacum than to N. plumbaginifolia, and many were indistinguishable from N. tabacum. It was found that 37 plants displayed one or two esterases characteristic of N. plumbaginifolia in addition to a full set of esterases from N. tabacum. Based on their esterases, we have classified these plants as somatic hybrids. However, irradiation has clearly reduced the amount of N. plumbaginifolia genetic material that they retain; 24 plants were found that had only N. tabacum esterases but that produced nopaline and were kanamycin resistant. Genomic DNA from several of these plants was probed by Southern blotting for the presence of the authentic neomycin phosphotransferase gene (kanamycin-resistance gene) - all were found to contain the gene. These plants were classified as asymmetric hybrids. Finally, 25 plants were regenerated that were kanamycin sensitive, negative for nopaline, and contained only N. tabacum esterases. All of the regenerated plants, including this final category, were male sterile. As transferring the N. plumbaginifolia cytoplasm to an N. tabacum nuclear background results in an alloplasmic form of male sterility, all of the plants regenerated in this study appear to be cybrids irrespective of their nuclear constitution. Chromosome analysis of the asymmetric hybrids showed that most of them contained one more chromosome than is normal for N. tabacum. The somatic hybrids examined all had several additional chromosomes. Although male sterile, the asymmetric hybrids were female fertile to varying degrees and were successfully backcrossed with N. tabacum. Analysis of the resultant F1 progeny indicated that the kanamycin-resistance gene from N. plumbaginifolia is partially unstable during meiosis, as would be expected for factors inherited on an unpaired chromosome. More... »

PAGES

718-726

References to SciGraph publications

  • 1982-12. Correction of nitrate reductase defect in auxotrophic plant cells through protoplast-mediated intergeneric gene transfers in MOLECULAR GENETICS AND GENOMICS
  • 1984-10. Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • 1981-03. Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids in THEORETICAL AND APPLIED GENETICS
  • 1986-11. Fusion-mediated transfer of triazine-resistant chloroplasts: Characterization of Nicotiana tabacum cybrid plants in MOLECULAR GENETICS AND GENOMICS
  • 1985-08. Electrical fusion for optimal formation of protoplast heterokaryons in Nicotiana in PLANTA
  • 1983-10. Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species in PLANT CELL REPORTS
  • 1975-07. Sexual transfer of specific genes without gametic fusion in NATURE
  • 1983-09. A heteroplasmic state induced by protoplast fusion is a necessary condition for detecting rearrangements in Nicotiana mitochondrial DNA in THEORETICAL AND APPLIED GENETICS
  • 1980-09. Intergeneric gene transfer mediated by plant protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • 1981-02. Uptake of isolated plant chromosomes by plant protoplasts in PLANTA
  • 1985-06. Culture of plant somatic hybrids following electrical fusion in THEORETICAL AND APPLIED GENETICS
  • Journal

    TITLE

    Theoretical and Applied Genetics

    ISSUE

    6

    VOLUME

    74

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00247548

    DOI

    http://dx.doi.org/10.1007/bf00247548

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1003604204

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24240331


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Florida State University", 
              "id": "https://www.grid.ac/institutes/grid.255986.5", 
              "name": [
                "Department of Biological Science and the Institute of Molecular Biophysics, Florida State University, 32306, Tallahassee, FL, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bates", 
            "givenName": "G. W.", 
            "id": "sg:person.010262554402.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010262554402.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Florida State University", 
              "id": "https://www.grid.ac/institutes/grid.255986.5", 
              "name": [
                "Department of Biological Science and the Institute of Molecular Biophysics, Florida State University, 32306, Tallahassee, FL, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hasenkampf", 
            "givenName": "C. A.", 
            "id": "sg:person.01051227312.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051227312.64"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Florida State University", 
              "id": "https://www.grid.ac/institutes/grid.255986.5", 
              "name": [
                "Department of Biological Science and the Institute of Molecular Biophysics, Florida State University, 32306, Tallahassee, FL, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Contolini", 
            "givenName": "C. L.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Florida State University", 
              "id": "https://www.grid.ac/institutes/grid.255986.5", 
              "name": [
                "Department of Biological Science and the Institute of Molecular Biophysics, Florida State University, 32306, Tallahassee, FL, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Piastuch", 
            "givenName": "W. C.", 
            "id": "sg:person.01067761105.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067761105.20"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/0005-2744(78)90363-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002663215"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2744(78)90363-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002663215"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/256310a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003800261", 
              "https://doi.org/10.1038/256310a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00425455", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005082789", 
              "https://doi.org/10.1007/bf00425455"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00264975", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006780108", 
              "https://doi.org/10.1007/bf00264975"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00264975", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006780108", 
              "https://doi.org/10.1007/bf00264975"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330037", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013927715", 
              "https://doi.org/10.1007/bf00330037"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00327919", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015515341", 
              "https://doi.org/10.1007/bf00327919"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0044-328x(76)80093-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024944233"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.bi.50.070181.002533", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025923897"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0304-4211(79)90062-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029603154"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033296365"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00304903", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038367797", 
              "https://doi.org/10.1007/bf00304903"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00387815", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038628550", 
              "https://doi.org/10.1007/bf00387815"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00387815", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038628550", 
              "https://doi.org/10.1007/bf00387815"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00269151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040500001", 
              "https://doi.org/10.1007/bf00269151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00269151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040500001", 
              "https://doi.org/10.1007/bf00269151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00430427", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044960746", 
              "https://doi.org/10.1007/bf00430427"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00395044", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046611196", 
              "https://doi.org/10.1007/bf00395044"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00395044", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046611196", 
              "https://doi.org/10.1007/bf00395044"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00251143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047565369", 
              "https://doi.org/10.1007/bf00251143"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00251143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047565369", 
              "https://doi.org/10.1007/bf00251143"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0074-7696(08)60324-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048933287"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.213.4510.907", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062523568"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.223.4635.496", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062528157"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.227.4691.1229", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062529900"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076549549", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077355999", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1537-2197.1960.tb07098.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1100719908"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1987-10", 
        "datePublishedReg": "1987-10-01", 
        "description": "Mesophyll protoplasts of a kanamycin-resistant, nopaline-positive Nicotiana plumbaginifolia seed line were inactivated by \u03b3-irradiation and electrically fused with unirradiated mesophyll protoplasts of N. tabacum. Hybrids were selected on kanamycin and regenerated. Genetic material from N. plumbaginifolia was detected in these plants by the following criteria: (1) morphology, (2) esterase isozyme profiles, and (3) the presence of nopaline in leaf extracts. All of the plants regenerated were morphologically more similar to N. tabacum than to N. plumbaginifolia, and many were indistinguishable from N. tabacum. It was found that 37 plants displayed one or two esterases characteristic of N. plumbaginifolia in addition to a full set of esterases from N. tabacum. Based on their esterases, we have classified these plants as somatic hybrids. However, irradiation has clearly reduced the amount of N. plumbaginifolia genetic material that they retain; 24 plants were found that had only N. tabacum esterases but that produced nopaline and were kanamycin resistant. Genomic DNA from several of these plants was probed by Southern blotting for the presence of the authentic neomycin phosphotransferase gene (kanamycin-resistance gene) - all were found to contain the gene. These plants were classified as asymmetric hybrids. Finally, 25 plants were regenerated that were kanamycin sensitive, negative for nopaline, and contained only N. tabacum esterases. All of the regenerated plants, including this final category, were male sterile. As transferring the N. plumbaginifolia cytoplasm to an N. tabacum nuclear background results in an alloplasmic form of male sterility, all of the plants regenerated in this study appear to be cybrids irrespective of their nuclear constitution. Chromosome analysis of the asymmetric hybrids showed that most of them contained one more chromosome than is normal for N. tabacum. The somatic hybrids examined all had several additional chromosomes. Although male sterile, the asymmetric hybrids were female fertile to varying degrees and were successfully backcrossed with N. tabacum. Analysis of the resultant F1 progeny indicated that the kanamycin-resistance gene from N. plumbaginifolia is partially unstable during meiosis, as would be expected for factors inherited on an unpaired chromosome. ", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00247548", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1135804", 
            "issn": [
              "0040-5752", 
              "1432-2242"
            ], 
            "name": "Theoretical and Applied Genetics", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "6", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "74"
          }
        ], 
        "name": "Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts", 
        "pagination": "718-726", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "112b65cca2c459bbdef4f9d13da1edbc3c39a27f86c70ecc0c8ce14eba97aaf8"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "24240331"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0145600"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00247548"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1003604204"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00247548", 
          "https://app.dimensions.ai/details/publication/pub.1003604204"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:52", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130805_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00247548"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00247548'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00247548'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00247548'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00247548'


     

    This table displays all metadata directly associated to this object as RDF triples.

    167 TRIPLES      21 PREDICATES      52 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00247548 schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author N7ee395aae9564c68a747d18a0257ff24
    4 schema:citation sg:pub.10.1007/bf00251143
    5 sg:pub.10.1007/bf00264975
    6 sg:pub.10.1007/bf00269151
    7 sg:pub.10.1007/bf00304903
    8 sg:pub.10.1007/bf00327919
    9 sg:pub.10.1007/bf00330037
    10 sg:pub.10.1007/bf00387815
    11 sg:pub.10.1007/bf00395044
    12 sg:pub.10.1007/bf00425455
    13 sg:pub.10.1007/bf00430427
    14 sg:pub.10.1038/256310a0
    15 https://app.dimensions.ai/details/publication/pub.1076549549
    16 https://app.dimensions.ai/details/publication/pub.1077355999
    17 https://doi.org/10.1002/j.1537-2197.1960.tb07098.x
    18 https://doi.org/10.1016/0005-2744(78)90363-7
    19 https://doi.org/10.1016/0304-4211(79)90062-2
    20 https://doi.org/10.1016/s0022-2836(75)80083-0
    21 https://doi.org/10.1016/s0044-328x(76)80093-1
    22 https://doi.org/10.1016/s0074-7696(08)60324-1
    23 https://doi.org/10.1126/science.213.4510.907
    24 https://doi.org/10.1126/science.223.4635.496
    25 https://doi.org/10.1126/science.227.4691.1229
    26 https://doi.org/10.1146/annurev.bi.50.070181.002533
    27 schema:datePublished 1987-10
    28 schema:datePublishedReg 1987-10-01
    29 schema:description Mesophyll protoplasts of a kanamycin-resistant, nopaline-positive Nicotiana plumbaginifolia seed line were inactivated by γ-irradiation and electrically fused with unirradiated mesophyll protoplasts of N. tabacum. Hybrids were selected on kanamycin and regenerated. Genetic material from N. plumbaginifolia was detected in these plants by the following criteria: (1) morphology, (2) esterase isozyme profiles, and (3) the presence of nopaline in leaf extracts. All of the plants regenerated were morphologically more similar to N. tabacum than to N. plumbaginifolia, and many were indistinguishable from N. tabacum. It was found that 37 plants displayed one or two esterases characteristic of N. plumbaginifolia in addition to a full set of esterases from N. tabacum. Based on their esterases, we have classified these plants as somatic hybrids. However, irradiation has clearly reduced the amount of N. plumbaginifolia genetic material that they retain; 24 plants were found that had only N. tabacum esterases but that produced nopaline and were kanamycin resistant. Genomic DNA from several of these plants was probed by Southern blotting for the presence of the authentic neomycin phosphotransferase gene (kanamycin-resistance gene) - all were found to contain the gene. These plants were classified as asymmetric hybrids. Finally, 25 plants were regenerated that were kanamycin sensitive, negative for nopaline, and contained only N. tabacum esterases. All of the regenerated plants, including this final category, were male sterile. As transferring the N. plumbaginifolia cytoplasm to an N. tabacum nuclear background results in an alloplasmic form of male sterility, all of the plants regenerated in this study appear to be cybrids irrespective of their nuclear constitution. Chromosome analysis of the asymmetric hybrids showed that most of them contained one more chromosome than is normal for N. tabacum. The somatic hybrids examined all had several additional chromosomes. Although male sterile, the asymmetric hybrids were female fertile to varying degrees and were successfully backcrossed with N. tabacum. Analysis of the resultant F1 progeny indicated that the kanamycin-resistance gene from N. plumbaginifolia is partially unstable during meiosis, as would be expected for factors inherited on an unpaired chromosome.
    30 schema:genre research_article
    31 schema:inLanguage en
    32 schema:isAccessibleForFree false
    33 schema:isPartOf N00876c4c7beb4004904b4aefb0628dfc
    34 N9d2486e080b047989ddab235709a7646
    35 sg:journal.1135804
    36 schema:name Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts
    37 schema:pagination 718-726
    38 schema:productId N054822fe88e14d6ea8beff231fe215cb
    39 N2d79fa9993ff407f8dc1fbbc53b82bbc
    40 Nee7939d27a4e463488240de73fbd28ec
    41 Nf0fb6545ae0041959e867a49689ff0f0
    42 Nf6eb42e931104a949eafd7897e95c12d
    43 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003604204
    44 https://doi.org/10.1007/bf00247548
    45 schema:sdDatePublished 2019-04-11T13:52
    46 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    47 schema:sdPublisher N024ded1db8974cae9d2a94980c5e1be9
    48 schema:url http://link.springer.com/10.1007/BF00247548
    49 sgo:license sg:explorer/license/
    50 sgo:sdDataset articles
    51 rdf:type schema:ScholarlyArticle
    52 N00876c4c7beb4004904b4aefb0628dfc schema:volumeNumber 74
    53 rdf:type schema:PublicationVolume
    54 N024ded1db8974cae9d2a94980c5e1be9 schema:name Springer Nature - SN SciGraph project
    55 rdf:type schema:Organization
    56 N054822fe88e14d6ea8beff231fe215cb schema:name nlm_unique_id
    57 schema:value 0145600
    58 rdf:type schema:PropertyValue
    59 N2d79fa9993ff407f8dc1fbbc53b82bbc schema:name readcube_id
    60 schema:value 112b65cca2c459bbdef4f9d13da1edbc3c39a27f86c70ecc0c8ce14eba97aaf8
    61 rdf:type schema:PropertyValue
    62 N5c003a78d59549beb372529421f77c6f schema:affiliation https://www.grid.ac/institutes/grid.255986.5
    63 schema:familyName Contolini
    64 schema:givenName C. L.
    65 rdf:type schema:Person
    66 N71cf5233f49c4d42ba941f5d103be2f5 rdf:first sg:person.01067761105.20
    67 rdf:rest rdf:nil
    68 N7ee395aae9564c68a747d18a0257ff24 rdf:first sg:person.010262554402.01
    69 rdf:rest Ne4ba056967614204a8caca7b0127baeb
    70 N9d2486e080b047989ddab235709a7646 schema:issueNumber 6
    71 rdf:type schema:PublicationIssue
    72 Ne4ba056967614204a8caca7b0127baeb rdf:first sg:person.01051227312.64
    73 rdf:rest Nfd20134970f34920a74ad21d7480ef91
    74 Nee7939d27a4e463488240de73fbd28ec schema:name doi
    75 schema:value 10.1007/bf00247548
    76 rdf:type schema:PropertyValue
    77 Nf0fb6545ae0041959e867a49689ff0f0 schema:name pubmed_id
    78 schema:value 24240331
    79 rdf:type schema:PropertyValue
    80 Nf6eb42e931104a949eafd7897e95c12d schema:name dimensions_id
    81 schema:value pub.1003604204
    82 rdf:type schema:PropertyValue
    83 Nfd20134970f34920a74ad21d7480ef91 rdf:first N5c003a78d59549beb372529421f77c6f
    84 rdf:rest N71cf5233f49c4d42ba941f5d103be2f5
    85 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    86 schema:name Biological Sciences
    87 rdf:type schema:DefinedTerm
    88 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    89 schema:name Genetics
    90 rdf:type schema:DefinedTerm
    91 sg:journal.1135804 schema:issn 0040-5752
    92 1432-2242
    93 schema:name Theoretical and Applied Genetics
    94 rdf:type schema:Periodical
    95 sg:person.010262554402.01 schema:affiliation https://www.grid.ac/institutes/grid.255986.5
    96 schema:familyName Bates
    97 schema:givenName G. W.
    98 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010262554402.01
    99 rdf:type schema:Person
    100 sg:person.01051227312.64 schema:affiliation https://www.grid.ac/institutes/grid.255986.5
    101 schema:familyName Hasenkampf
    102 schema:givenName C. A.
    103 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051227312.64
    104 rdf:type schema:Person
    105 sg:person.01067761105.20 schema:affiliation https://www.grid.ac/institutes/grid.255986.5
    106 schema:familyName Piastuch
    107 schema:givenName W. C.
    108 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067761105.20
    109 rdf:type schema:Person
    110 sg:pub.10.1007/bf00251143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047565369
    111 https://doi.org/10.1007/bf00251143
    112 rdf:type schema:CreativeWork
    113 sg:pub.10.1007/bf00264975 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006780108
    114 https://doi.org/10.1007/bf00264975
    115 rdf:type schema:CreativeWork
    116 sg:pub.10.1007/bf00269151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040500001
    117 https://doi.org/10.1007/bf00269151
    118 rdf:type schema:CreativeWork
    119 sg:pub.10.1007/bf00304903 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038367797
    120 https://doi.org/10.1007/bf00304903
    121 rdf:type schema:CreativeWork
    122 sg:pub.10.1007/bf00327919 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015515341
    123 https://doi.org/10.1007/bf00327919
    124 rdf:type schema:CreativeWork
    125 sg:pub.10.1007/bf00330037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013927715
    126 https://doi.org/10.1007/bf00330037
    127 rdf:type schema:CreativeWork
    128 sg:pub.10.1007/bf00387815 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038628550
    129 https://doi.org/10.1007/bf00387815
    130 rdf:type schema:CreativeWork
    131 sg:pub.10.1007/bf00395044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046611196
    132 https://doi.org/10.1007/bf00395044
    133 rdf:type schema:CreativeWork
    134 sg:pub.10.1007/bf00425455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005082789
    135 https://doi.org/10.1007/bf00425455
    136 rdf:type schema:CreativeWork
    137 sg:pub.10.1007/bf00430427 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044960746
    138 https://doi.org/10.1007/bf00430427
    139 rdf:type schema:CreativeWork
    140 sg:pub.10.1038/256310a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003800261
    141 https://doi.org/10.1038/256310a0
    142 rdf:type schema:CreativeWork
    143 https://app.dimensions.ai/details/publication/pub.1076549549 schema:CreativeWork
    144 https://app.dimensions.ai/details/publication/pub.1077355999 schema:CreativeWork
    145 https://doi.org/10.1002/j.1537-2197.1960.tb07098.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1100719908
    146 rdf:type schema:CreativeWork
    147 https://doi.org/10.1016/0005-2744(78)90363-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002663215
    148 rdf:type schema:CreativeWork
    149 https://doi.org/10.1016/0304-4211(79)90062-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029603154
    150 rdf:type schema:CreativeWork
    151 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
    152 rdf:type schema:CreativeWork
    153 https://doi.org/10.1016/s0044-328x(76)80093-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024944233
    154 rdf:type schema:CreativeWork
    155 https://doi.org/10.1016/s0074-7696(08)60324-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048933287
    156 rdf:type schema:CreativeWork
    157 https://doi.org/10.1126/science.213.4510.907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062523568
    158 rdf:type schema:CreativeWork
    159 https://doi.org/10.1126/science.223.4635.496 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062528157
    160 rdf:type schema:CreativeWork
    161 https://doi.org/10.1126/science.227.4691.1229 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062529900
    162 rdf:type schema:CreativeWork
    163 https://doi.org/10.1146/annurev.bi.50.070181.002533 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025923897
    164 rdf:type schema:CreativeWork
    165 https://www.grid.ac/institutes/grid.255986.5 schema:alternateName Florida State University
    166 schema:name Department of Biological Science and the Institute of Molecular Biophysics, Florida State University, 32306, Tallahassee, FL, USA
    167 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...