Restriction endonuclease digestion of meiotic chromosomes of Allium subvillosum L. View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-01

AUTHORS

J. Fernandez-Piqueras, R. Lozano, C. Sentis, M. Ruiz Rejón

ABSTRACT

Meiotic chromosomes of the liliaceous plant Allium subvillosum were characterized by means of digestion with the restriction endonucleases (REs) Hae III and Msp I followed in some cases by treatments with proteinase K or nuclease S1. Both REs are capable of digesting euchromatin, giving a C-like banding pattern. Something similar can be observed when chromosomes are digested with the two restriction endonucleases followed by treatments with proteinase K. By contrast, heterochromatic regions can be digested only after sequential treatments using Hae III plus nuclease S1. These results are discussed in relation to the structural organization of plant meiotic chromosomes as well as the special conformation of plant heterochromatin. More... »

PAGES

165-168

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00240556

DOI

http://dx.doi.org/10.1007/bf00240556

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015307170


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