The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-08

AUTHORS

Dennis Keefe, Ursula Hinz, Frederick Meins

ABSTRACT

We have studied the effect of ethylene on the localization of the basic isoforms of glucan endo-1,3-β-glucosidase (β-1,3-glucanase, EC 3.2.1.39) and endo-chitinase (chitinase, EC 3.2.1.14) in leaves of Nicotiana tabacum L. cv. Havana 425. Comparisons of the enzyme contents of the lower epidermis of the leaf, leaf expiants with the lower epidermis removed, and intercellular wash fluid indicate that both enzymes are localized inside epidermal cells of untreated leaves. Ethylene treatment (20 μl·l-1, 4d) induced a marked -10- to 30-fold-coordinated accumulation of the enzymes. This was due primarily to induction of the basic isoforms inside chlorenchyma cells of the leaf interior. The localization of basic β-1,3-glucanase was confirmed by immunofluorescence histochemistry and immunogold cytochemistry. Immunolabelling was confined to electron-dense bodies of the cell vacuole. No extracellular immunolabelling was detected in control or ethylene-treated leaves. We conclude that ethylene changes the cell-type-specific distribution but not the intracellular compartmentation of the two enzymes. These results support the generalization that basic isoforms of chitinase and β-1,3-glucanase are intracellular whereas the acidic isoforms are secreted into the extracellular space. More... »

PAGES

43-51

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00239982

DOI

http://dx.doi.org/10.1007/bf00239982

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037145218

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24196998


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland", 
          "id": "http://www.grid.ac/institutes/grid.482245.d", 
          "name": [
            "Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Keefe", 
        "givenName": "Dennis", 
        "id": "sg:person.01053747700.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053747700.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland", 
          "id": "http://www.grid.ac/institutes/grid.482245.d", 
          "name": [
            "Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hinz", 
        "givenName": "Ursula", 
        "id": "sg:person.0735056122.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735056122.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland", 
          "id": "http://www.grid.ac/institutes/grid.482245.d", 
          "name": [
            "Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Meins", 
        "givenName": "Frederick", 
        "id": "sg:person.01136614547.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136614547.28"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00394536", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024658349", 
          "https://doi.org/10.1007/bf00394536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-7091-6950-6_11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038549592", 
          "https://doi.org/10.1007/978-3-7091-6950-6_11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00028772", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037550631", 
          "https://doi.org/10.1007/bf00028772"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02418757", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010357962", 
          "https://doi.org/10.1007/bf02418757"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00014914", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017610247", 
          "https://doi.org/10.1007/bf00014914"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/324365a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013176934", 
          "https://doi.org/10.1038/324365a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00959522", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045053566", 
          "https://doi.org/10.1007/bf00959522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00402956", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044731145", 
          "https://doi.org/10.1007/bf00402956"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4615-7084-4_5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053075417", 
          "https://doi.org/10.1007/978-1-4615-7084-4_5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00391416", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008336630", 
          "https://doi.org/10.1007/bf00391416"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00397892", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044440904", 
          "https://doi.org/10.1007/bf00397892"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00398668", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014746209", 
          "https://doi.org/10.1007/bf00398668"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1990-08", 
    "datePublishedReg": "1990-08-01", 
    "description": "We have studied the effect of ethylene on the localization of the basic isoforms of glucan endo-1,3-\u03b2-glucosidase (\u03b2-1,3-glucanase, EC 3.2.1.39) and endo-chitinase (chitinase, EC 3.2.1.14) in leaves of Nicotiana tabacum L. cv. Havana 425. Comparisons of the enzyme contents of the lower epidermis of the leaf, leaf expiants with the lower epidermis removed, and intercellular wash fluid indicate that both enzymes are localized inside epidermal cells of untreated leaves. Ethylene treatment (20 \u03bcl\u00b7l-1, 4d) induced a marked -10- to 30-fold-coordinated accumulation of the enzymes. This was due primarily to induction of the basic isoforms inside chlorenchyma cells of the leaf interior. The localization of basic \u03b2-1,3-glucanase was confirmed by immunofluorescence histochemistry and immunogold cytochemistry. Immunolabelling was confined to electron-dense bodies of the cell vacuole. No extracellular immunolabelling was detected in control or ethylene-treated leaves. We conclude that ethylene changes the cell-type-specific distribution but not the intracellular compartmentation of the two enzymes. These results support the generalization that basic isoforms of chitinase and \u03b2-1,3-glucanase are intracellular whereas the acidic isoforms are secreted into the extracellular space.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00239982", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "182"
      }
    ], 
    "keywords": [
      "basic isoforms", 
      "effect of ethylene", 
      "intercellular wash fluids", 
      "Nicotiana tabacum L. cv", 
      "lower epidermis", 
      "ethylene-treated leaves", 
      "glucan endo", 
      "coordinated accumulation", 
      "chlorenchyma cells", 
      "leaf expiants", 
      "leaf interior", 
      "tobacco leaves", 
      "intracellular localization", 
      "intracellular compartmentation", 
      "cell vacuoles", 
      "epidermal cells", 
      "untreated leaves", 
      "electron-dense bodies", 
      "ethylene treatment", 
      "leaves", 
      "glucanase", 
      "acidic isoforms", 
      "L. cv", 
      "isoforms", 
      "\u03b2-glucosidase", 
      "extracellular space", 
      "enzyme", 
      "chitinase", 
      "enzyme content", 
      "localization", 
      "specific distribution", 
      "cells", 
      "epidermis", 
      "compartmentation", 
      "vacuoles", 
      "ethylene", 
      "expiants", 
      "cytochemistry", 
      "immunofluorescence histochemistry", 
      "induction", 
      "endo", 
      "accumulation", 
      "cv", 
      "wash fluid", 
      "immunolabelling", 
      "histochemistry", 
      "effect", 
      "interior", 
      "body", 
      "content", 
      "distribution", 
      "control", 
      "treatment", 
      "comparison", 
      "results", 
      "fluid", 
      "space", 
      "generalization"
    ], 
    "name": "The effect of ethylene on the cell-type-specific and intracellular localization of \u03b2-1,3-glucanase and chitinase in tobacco leaves", 
    "pagination": "43-51", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037145218"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00239982"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24196998"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00239982", 
      "https://app.dimensions.ai/details/publication/pub.1037145218"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:29", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_238.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00239982"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00239982'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00239982'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00239982'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00239982'


 

This table displays all metadata directly associated to this object as RDF triples.

185 TRIPLES      21 PREDICATES      97 URIs      76 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00239982 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Ne34c54486d8c431b832ff6b2a15ca5a0
4 schema:citation sg:pub.10.1007/978-1-4615-7084-4_5
5 sg:pub.10.1007/978-3-7091-6950-6_11
6 sg:pub.10.1007/bf00014914
7 sg:pub.10.1007/bf00028772
8 sg:pub.10.1007/bf00391416
9 sg:pub.10.1007/bf00394536
10 sg:pub.10.1007/bf00397892
11 sg:pub.10.1007/bf00398668
12 sg:pub.10.1007/bf00402956
13 sg:pub.10.1007/bf00959522
14 sg:pub.10.1007/bf02418757
15 sg:pub.10.1038/227680a0
16 sg:pub.10.1038/324365a0
17 schema:datePublished 1990-08
18 schema:datePublishedReg 1990-08-01
19 schema:description We have studied the effect of ethylene on the localization of the basic isoforms of glucan endo-1,3-β-glucosidase (β-1,3-glucanase, EC 3.2.1.39) and endo-chitinase (chitinase, EC 3.2.1.14) in leaves of Nicotiana tabacum L. cv. Havana 425. Comparisons of the enzyme contents of the lower epidermis of the leaf, leaf expiants with the lower epidermis removed, and intercellular wash fluid indicate that both enzymes are localized inside epidermal cells of untreated leaves. Ethylene treatment (20 μl·l-1, 4d) induced a marked -10- to 30-fold-coordinated accumulation of the enzymes. This was due primarily to induction of the basic isoforms inside chlorenchyma cells of the leaf interior. The localization of basic β-1,3-glucanase was confirmed by immunofluorescence histochemistry and immunogold cytochemistry. Immunolabelling was confined to electron-dense bodies of the cell vacuole. No extracellular immunolabelling was detected in control or ethylene-treated leaves. We conclude that ethylene changes the cell-type-specific distribution but not the intracellular compartmentation of the two enzymes. These results support the generalization that basic isoforms of chitinase and β-1,3-glucanase are intracellular whereas the acidic isoforms are secreted into the extracellular space.
20 schema:genre article
21 schema:isAccessibleForFree false
22 schema:isPartOf Na3ebafe54d25447898ebd564fffd84b6
23 Nbdb424f51cb94a0e8282edc179d8b6a3
24 sg:journal.1054035
25 schema:keywords L. cv
26 Nicotiana tabacum L. cv
27 accumulation
28 acidic isoforms
29 basic isoforms
30 body
31 cell vacuoles
32 cells
33 chitinase
34 chlorenchyma cells
35 comparison
36 compartmentation
37 content
38 control
39 coordinated accumulation
40 cv
41 cytochemistry
42 distribution
43 effect
44 effect of ethylene
45 electron-dense bodies
46 endo
47 enzyme
48 enzyme content
49 epidermal cells
50 epidermis
51 ethylene
52 ethylene treatment
53 ethylene-treated leaves
54 expiants
55 extracellular space
56 fluid
57 generalization
58 glucan endo
59 glucanase
60 histochemistry
61 immunofluorescence histochemistry
62 immunolabelling
63 induction
64 intercellular wash fluids
65 interior
66 intracellular compartmentation
67 intracellular localization
68 isoforms
69 leaf expiants
70 leaf interior
71 leaves
72 localization
73 lower epidermis
74 results
75 space
76 specific distribution
77 tobacco leaves
78 treatment
79 untreated leaves
80 vacuoles
81 wash fluid
82 β-glucosidase
83 schema:name The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves
84 schema:pagination 43-51
85 schema:productId N762cf7ff251449d8ac816172e9a48b78
86 N83b4744fb71d4672815d01ff6803f6de
87 Ne5b348e9dda24269b782dc26b96c5216
88 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037145218
89 https://doi.org/10.1007/bf00239982
90 schema:sdDatePublished 2022-10-01T06:29
91 schema:sdLicense https://scigraph.springernature.com/explorer/license/
92 schema:sdPublisher Nde6fe95e52df4c0ea219cacdd7711556
93 schema:url https://doi.org/10.1007/bf00239982
94 sgo:license sg:explorer/license/
95 sgo:sdDataset articles
96 rdf:type schema:ScholarlyArticle
97 N32d0b59210a84625a3b3ce6c35cea7e6 rdf:first sg:person.0735056122.72
98 rdf:rest N715a4b850493441fa8d628732e07d4b2
99 N715a4b850493441fa8d628732e07d4b2 rdf:first sg:person.01136614547.28
100 rdf:rest rdf:nil
101 N762cf7ff251449d8ac816172e9a48b78 schema:name pubmed_id
102 schema:value 24196998
103 rdf:type schema:PropertyValue
104 N83b4744fb71d4672815d01ff6803f6de schema:name dimensions_id
105 schema:value pub.1037145218
106 rdf:type schema:PropertyValue
107 Na3ebafe54d25447898ebd564fffd84b6 schema:issueNumber 1
108 rdf:type schema:PublicationIssue
109 Nbdb424f51cb94a0e8282edc179d8b6a3 schema:volumeNumber 182
110 rdf:type schema:PublicationVolume
111 Nde6fe95e52df4c0ea219cacdd7711556 schema:name Springer Nature - SN SciGraph project
112 rdf:type schema:Organization
113 Ne34c54486d8c431b832ff6b2a15ca5a0 rdf:first sg:person.01053747700.31
114 rdf:rest N32d0b59210a84625a3b3ce6c35cea7e6
115 Ne5b348e9dda24269b782dc26b96c5216 schema:name doi
116 schema:value 10.1007/bf00239982
117 rdf:type schema:PropertyValue
118 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
119 schema:name Biological Sciences
120 rdf:type schema:DefinedTerm
121 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
122 schema:name Biochemistry and Cell Biology
123 rdf:type schema:DefinedTerm
124 sg:journal.1054035 schema:issn 0032-0935
125 1432-2048
126 schema:name Planta
127 schema:publisher Springer Nature
128 rdf:type schema:Periodical
129 sg:person.01053747700.31 schema:affiliation grid-institutes:grid.482245.d
130 schema:familyName Keefe
131 schema:givenName Dennis
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053747700.31
133 rdf:type schema:Person
134 sg:person.01136614547.28 schema:affiliation grid-institutes:grid.482245.d
135 schema:familyName Meins
136 schema:givenName Frederick
137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136614547.28
138 rdf:type schema:Person
139 sg:person.0735056122.72 schema:affiliation grid-institutes:grid.482245.d
140 schema:familyName Hinz
141 schema:givenName Ursula
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735056122.72
143 rdf:type schema:Person
144 sg:pub.10.1007/978-1-4615-7084-4_5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053075417
145 https://doi.org/10.1007/978-1-4615-7084-4_5
146 rdf:type schema:CreativeWork
147 sg:pub.10.1007/978-3-7091-6950-6_11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038549592
148 https://doi.org/10.1007/978-3-7091-6950-6_11
149 rdf:type schema:CreativeWork
150 sg:pub.10.1007/bf00014914 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017610247
151 https://doi.org/10.1007/bf00014914
152 rdf:type schema:CreativeWork
153 sg:pub.10.1007/bf00028772 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037550631
154 https://doi.org/10.1007/bf00028772
155 rdf:type schema:CreativeWork
156 sg:pub.10.1007/bf00391416 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008336630
157 https://doi.org/10.1007/bf00391416
158 rdf:type schema:CreativeWork
159 sg:pub.10.1007/bf00394536 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024658349
160 https://doi.org/10.1007/bf00394536
161 rdf:type schema:CreativeWork
162 sg:pub.10.1007/bf00397892 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044440904
163 https://doi.org/10.1007/bf00397892
164 rdf:type schema:CreativeWork
165 sg:pub.10.1007/bf00398668 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014746209
166 https://doi.org/10.1007/bf00398668
167 rdf:type schema:CreativeWork
168 sg:pub.10.1007/bf00402956 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044731145
169 https://doi.org/10.1007/bf00402956
170 rdf:type schema:CreativeWork
171 sg:pub.10.1007/bf00959522 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045053566
172 https://doi.org/10.1007/bf00959522
173 rdf:type schema:CreativeWork
174 sg:pub.10.1007/bf02418757 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010357962
175 https://doi.org/10.1007/bf02418757
176 rdf:type schema:CreativeWork
177 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
178 https://doi.org/10.1038/227680a0
179 rdf:type schema:CreativeWork
180 sg:pub.10.1038/324365a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013176934
181 https://doi.org/10.1038/324365a0
182 rdf:type schema:CreativeWork
183 grid-institutes:grid.482245.d schema:alternateName Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland
184 schema:name Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland
185 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...