Development of immunologically identified brain cells in culture: Quantitative aspects View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1983-12

AUTHORS

L. Bologa, R. Joubert, J.-C. Bisconte, S. Margules, M.-A. Deugnier, C. Derbin, N. Herschkowitz

ABSTRACT

Mechanically dissociated brain cells of 14 and 18-day-old mouse embryos and of mouse neonates were cultured for 3 weeks. Neurons, oligodendrocytes and astrocytes were identified at the 7th, 14th and 21st day in vitro by staining the cultures using the indirect immunoperoxidase technique with antisera directed against neuron specific enolase, galactocerebroside, myelin basic protein and glial fibrillary acidic protein. The number of neurons and oligodendrocytes was higher in embryonic cultures than in neonate cultures. The expression of some antigens was also different in the two types of culture. Our results indicate that the development of brain cells in mechanically dissociated brain cell cultures depends on the age of the animal at the time of plating. More... »

PAGES

163-167

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00239408

DOI

http://dx.doi.org/10.1007/bf00239408

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037546639

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6370712


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