Phosphinothricin stimulates somatic embryogenesis in grape (Vitis sp. L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-03

AUTHORS

Dominique Hébert-Soulé, Julie R. Kikkert, Bruce I. Reisch

ABSTRACT

The effect of phosphinothricin concentration on embryo production from an embryogenic callus of 'Chancellor' (Vitis L. complex interspecific hybrid) was tested. Embryogenic callus was cultured on medium supplemented with nine phosphinothricin concentrations (0, 0.1, 0.5, 1, 1.5, 2, 3, 5, and 10 mg/l). The highest number of embryos per plate was observed at 0.5 mg/l phosphinothricin. The use of phosphinothricin to stimulate embryo production did not affect embryo germination and plantlet formation. Three germination techniques were compared. Embryo dehydration or growth on Transfergelsolidified medium gave higher germination rates than chilling treatments. Most germinated somatic embryos produced secondary embryos from the hypocotyl after a few weeks of culture. Regardless of the germination technique, the plantlet conversion rate was very low. More... »

PAGES

380-384

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00238601

DOI

http://dx.doi.org/10.1007/bf00238601

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018440214

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24185337


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