Plant regeneration from stem cortex expiant and protoplast cultures of Brassica juncea (mustard) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-11

AUTHORS

A.-C. Bonfils, A. Sproule, J. A. Webb, W. A. Keller

ABSTRACT

Plant regeneration from stem cortex explants of 13 genotypes of Brassica juncea was assessed. Regeneration was strongly affected by genotype, as up to 50.6 shoots were produced per 100 calli of the most responsive line (Blaze), whereas no shoots were obtained from less responsive lines (Zeml, Vniimk351). Blaze was chosen for B. juncea stem cortex protoplast isolation. After one week of culture, 11-14% of the cells had divided, and about 0.002% produced 1-2 mm colonies within 6 weeks. Up to 7% of these colonies gave rise to shoots upon transfer to plant regeneration medium. More... »

PAGES

614-617

Journal

TITLE

Plant Cell Reports

ISSUE

12

VOLUME

11

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00236384

DOI

http://dx.doi.org/10.1007/bf00236384

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021289146

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24213363


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