In vivo and in vitro studies on the appearance of LHRH neurons in the hypothalamus of perinatal rats View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-12

AUTHORS

Shigeo Daikoku, Hitoshi Kawano, Hideo Matsumura, Shiro Saito

ABSTRACT

Ontogenetic development of LHRH-containing neurons was studied by fluorescence and enzyme immunohistochemistry in rats. In in vitro studies, the tissues of the septal-chiasmatic and mediobasal hypothalamic areas of fetal rats on day 16.5 or 18.5 of gestation were trypsinized separately for dissociation of the neural cells, and cultured for several days. Immunopositive reaction against LHRH was first detected in nerve cells derived from both areas of the hypothalamus of the fetuses on days 16.5 and 18.5 of gestation, after 8 and 6 days culture, respectively. The cells were small, and seemed to be bipolar in morphology indicating an axon and arborized dendrites. Immunopositive material occurred in the cell soma as well as in the cellular processes. In in vivo studies, immunopositive material, possibly deposited in nerve fibers, appeared first in OVLT and simultaneously in the external layer of the median eminence of fetuses on day 20.5 of gestation. The immunoreactive fibers increased in number in both parts with development, especially after birth in the median eminence. No immunopositive material was detected within any neural cell bodies nor in the cytoplasm of any ependymal cells. More... »

PAGES

433-445

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00236164

DOI

http://dx.doi.org/10.1007/bf00236164

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048750162

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/365346


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