Regeneration of fertile plants from protoplasts of soybean (Glycine max L. Merr.): genotypic differences in culture response View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-06

AUTHORS

Sarwan K. Dhir, Seema Dhir, Jack M. Widholm

ABSTRACT

Fourteen soybean (Glycine max L.) genotypes were evaluated for their regenerability from protoplasts using a procedure previously descibed for the cultivar Clark 63. Protoplasts were isolated from immature cotyledon tissue and were cultured in liquid or agarose gelled KP8, MS or B5 medium with different sugars. Significant differences were observed in plating efficiency, which was as high as 63% in Jack and A-2396, and as low as 38% in X-3337. Upon regular dilution with K8 medium, 1-2 mm diameter colonies were formed in 5-6 weeks with all the genotypes tested. These colonies were then transferred onto MSB (MS salts; Murashige & Skoog, 1962 + B5 organics; Gamborg et al., 1968) medium with 0.5 mg l(-1) each of 2,4-D, BA and KN and 500 mg l(-1) CH for further growth. Once the colonies had become green, compact and nodular, and were 8-10 mm in size, they were transferred to regeneration medium. Upon regular subculturing, calli of six genotypes; A-2396, Chamberlain, Heilong-26, Jack, Resnick and XP-3015 developed shoots, with the regeneration frequency being highest 27% in Jack (52 calli out of 192 produced 8-12 shoots). The regenerated shoots from different genotypes were elongated and rooted. So far, sixty three complete plants have been obtained, including twelve A-2396, nineteen Chamberlain, fifteen Jack, nine Resnick and eight XP-3015. A total of thirty five plants have been transplanted into pots in the greenhouse. Sixteen have set seeds and others are producing flowers and pods. More... »

PAGES

285-289

References to SciGraph publications

  • 1991-07. Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.) in PLANT CELL REPORTS
  • 1988-08. Plant regeneration from protoplasts of soybean (Glycine max L.) in PLANT CELL REPORTS
  • 1977-01. Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca in MOLECULAR GENETICS AND GENOMICS
  • 1988-12. Plant regeneration from protoplasts of indica rice: genotypic differences in culture response in THEORETICAL AND APPLIED GENETICS
  • 1991-06. Factors affecting transient gene expression in electroporated Glycine max protoplasts in PLANT CELL REPORTS
  • 1991-05. Plantlet regeneration from immature cotyledon protoplasts of soybean (Glycine max L.) in PLANT CELL REPORTS
  • 1991-06. Regeneration of transformed shoots from electroporated soybean (Glycine max (L.) Merr.) protoplasts in PLANT CELL REPORTS
  • 1986-04. Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.) in PLANT CELL REPORTS
  • 1989-11. Genotype-dependent whole plant regeneration from protoplasts of red clover (Trifolium pratense L.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1987-06. Regeneration of leaf mesophyll protoplasts of tomato cultivars (L. esculentum): factors important for efficient protoplast culture and plant regeneration in PLANT CELL REPORTS
  • 1985-01. Protoplast culture and plant regeneration in Glycine canescens F.J. Herm in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1983-10. Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species in PLANT CELL REPORTS
  • 1988-07. Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes in MOLECULAR GENETICS AND GENOMICS
  • 1989-06. Protoplast regeneration and organogenesis from pea protoplasts in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 1990-04. A protoplast-to-plant system in chrysanthemum: differential responses among several commercial clones in PLANT CELL REPORTS
  • 1989-02. Plant regeneration of wildGlycine species from suspension culture-derived protoplasts in PLANT CELL REPORTS
  • Journal

    TITLE

    Plant Cell Reports

    ISSUE

    5-6

    VOLUME

    11

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00235083

    DOI

    http://dx.doi.org/10.1007/bf00235083

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1023466084

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24203141


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