Protoplast culture and plant regeneration of several species in the genus Dianthus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-06

AUTHORS

Masaru Nakano, Masahiro Mii

ABSTRACT

Seventeen cultivars belonging to the genus Dianthus were examined for protoplast isolation, culture and shoot regeneration under the same conditions. These included D. caryophyllus, D. chinensis, D. barbatus, D. plumarius, D. superbus and D. japonicus as well as interspecific hybrid cultivars (D. caryophyllus x D. chinensis and D. chinensis x D. barbatus). In all cultivars, viable protoplasts were isolated at high yields from leaves of axenic shoot cultures and some of these protoplasts divided and formed colonies. However, shoot regeneration frequencies were markedly different among the species. High frequency shoot regeneration was obtained from D. chinensis and interspecific hybrid cultivars, while only low frequency or no shoot regeneration was obtained from other species. More... »

PAGES

225-228

Journal

TITLE

Plant Cell Reports

ISSUE

5-6

VOLUME

11

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00235070

DOI

http://dx.doi.org/10.1007/bf00235070

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010552176

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24203128


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