Green fluorescent protein: an in vivo reporter of plant gene expression View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-04

AUTHORS

Randall P. Niedz, Michael R. Sussman, John S. Satterlee

ABSTRACT

Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light. More... »

PAGES

403-406

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00234043

DOI

http://dx.doi.org/10.1007/bf00234043

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024569897

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24185445


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