Somatic hybridization between Citrus reticulata and Citropsis gabunensis through electrofusion View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-06

AUTHORS

Jing-Tian Ling, M. Iwamasa

ABSTRACT

Protoplasts from embryogenic calli of Citrus reticulata Blanco cv. Ponkan and Citropsis gabunensis (Engl.) Swing. & M. Kell (Cabon Cherry Orange), were isolated and fused using electric current. Maximum fusion frequency was obtained with AC at 75 kV/cm (1.0 MHz) for 15 s, followed by DC square-wave pulses at 1.25 kV/cm for 40 μs. Fusion-treated protoplasts were cultured on MT medium containing no growth regulators, solidified with 0.6% Bacto Difco agar. Protoplast-derived calli were proliferated on MT medium containing 1 mg/l zeatin and 0.9% agar. A total of 31 lines of somatic hybrid calli were obtained by screening on the basis of chromosome count and isozyme analysis. The somatic hybrids were tetraploid (2n=36). Plants were regenerated from the calli via somatic embryogenesis. The somatic hybrid plants exhibited morphological characteristics intermediate to the parental plants. More... »

PAGES

493-497

Journal

TITLE

Plant Cell Reports

ISSUE

9

VOLUME

13

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232943

DOI

http://dx.doi.org/10.1007/bf00232943

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051390872

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24194127


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