A simple, versatile feeder layer system for Brassica oleracea protoplast culture View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-10

AUTHORS

Thomas W. Walters, Elizabeth D. Earle

ABSTRACT

Protoplasts from cauliflower (Brassica oleracea ssp. botrytis) and broccoli (ssp. italica) leaves and hypocotyls were successfully cultured on membrane filters over a feeder layer of cells from a B. campestris suspension culture. Cells from rice, tomato and tobacco suspensions were not as effective as the B. campestris cells. Plants were recovered from protoplasts of previously recalcitrant Brassica genotypes. Protoplasts cultured in low numbers (10-100) on the feeder layer divided and formed colonies capable of plant regeneration, as did fused protoplasts. More... »

PAGES

316-319

References to SciGraph publications

  • 1985-02. A simple culture method for Brassica hypototyl protoplasts in PLANT CELL REPORTS
  • 1988-08. Transfer and segregation of triazine tolerant chloroplasts in Brassica napus L. in THEORETICAL AND APPLIED GENETICS
  • 1985-12. Plant regeneration from mesophyll protoplast culture of cabbage (Brassica oleracea var ‘capitata’) in THEORETICAL AND APPLIED GENETICS
  • 1986-04. A rapid and efficient alternative procedure for the regeneration of plants from hypocotyl protoplasts of Brassica napus in PLANT CELL REPORTS
  • 1986-12. The introduction of CMS mitochondria to triazine tolerant Brassica napus L., var. “Regent”, by micromanipulation of individual heterokaryons in PLANT CELL REPORTS
  • 1988-01. Increased totipotency of protoplasts from Brassica oleracea plants previously regenerated in tissue culture in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1983-06. Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana in THEORETICAL AND APPLIED GENETICS
  • 1986-02. Plant regeneration from leaf protoplasts of Brassica oleracea var. italica CV Green Comet broccoli in PLANT CELL REPORTS
  • 1987-01. Plant regeneration from stem cortex protoplasts of Brassica napus in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1989-01. Growth and shoot formation in protoplast-derived calli of Brassica oleracea ssp. acephala and ssp. capitata in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1987-04. Individual selection, culture and manipulation of higher plant cells in THEORETICAL AND APPLIED GENETICS
  • 1983-08. Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • Journal

    TITLE

    Plant Cell Reports

    ISSUE

    6

    VOLUME

    9

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00232859

    DOI

    http://dx.doi.org/10.1007/bf00232859

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1046603921

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24226941


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