Ontology type: schema:ScholarlyArticle
1990-10
AUTHORSThomas W. Walters, Elizabeth D. Earle
ABSTRACTProtoplasts from cauliflower (Brassica oleracea ssp. botrytis) and broccoli (ssp. italica) leaves and hypocotyls were successfully cultured on membrane filters over a feeder layer of cells from a B. campestris suspension culture. Cells from rice, tomato and tobacco suspensions were not as effective as the B. campestris cells. Plants were recovered from protoplasts of previously recalcitrant Brassica genotypes. Protoplasts cultured in low numbers (10-100) on the feeder layer divided and formed colonies capable of plant regeneration, as did fused protoplasts. More... »
PAGES316-319
http://scigraph.springernature.com/pub.10.1007/bf00232859
DOIhttp://dx.doi.org/10.1007/bf00232859
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/24226941
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