Transformation of cultured cells of Chenopodium quinoa by binary vectors that carry a fragment of DNA from the virulence region ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-10

AUTHORS

Toshihiko Komari

ABSTRACT

A 15.2-kb KpnI fragment from the virulence region of pTiBo542, the Ti plasmid harbored by Agrobacterium tumefaciens strain A281, was introduced into binary vectors. The fragment contained the virB, virC and virG genes, and it is known to have the ability to increase the virulence of strains of A. tumefaciens. The strains of A. tumefaciens that carried the resulting plasmids were able to transform cells in a suspension culture of Chenopodium quinoa Willd cells which were not transformable by common vectors. Although the sizes of the plasmids was very large, a foreign segment of DNA was introduced into one of the plasmids by homologous recombination in A. tumefaciens cells, and the segment was subsequently transferred to plant cells. More... »

PAGES

303-306

Journal

TITLE

Plant Cell Reports

ISSUE

6

VOLUME

9

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232856

DOI

http://dx.doi.org/10.1007/bf00232856

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024486599

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24226938


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