Purification of mitochondria and mitochondrial nucleic acids from embryogenic suspension cultures of a gymnosperm, Larix x leptoeuropaea View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-05

AUTHORS

Raman Kumar, Marie-Anne Lelu, Ian Small

ABSTRACT

After a number of attempts to isolate mitochondria from different conifer tissues, embryogenic suspension cultures of hybrid larch (Larix x leptoeuropaea) were developed which enabled the purification of mitochondria using slight modifications to standard techniques. The mitochondrial purity was verified by analysis of the mitochondrial RNA, DNA and proteins. The larch mitochondrial genome size is surprisingly large (> 1000 kbp) and the polypeptide pattern differs greatly from those of wheat or potato mitochondria, suggesting that valuable evolutionary insights will be gained from comparisons between gymnosperm and angiosperm mitochondria. The ease with which embryogenic conifer suspensions can be initiated and used for mitochondrial purification implies that they will be the material of choice for future studies of this type. More... »

PAGES

534-538

Journal

TITLE

Plant Cell Reports

ISSUE

8

VOLUME

14

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232790

DOI

http://dx.doi.org/10.1007/bf00232790

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047138586

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24185527


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