Somatic embryogenesis from integument (perisperm) cultures of coffee View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-07-01

AUTHORS

H. L. Sreenath, H. M. Shanta, K. Harinath Babu, M. M. Naidu

ABSTRACT

Somatic embryogenesis was induced in integument (perisperm) cultures of C x R hybrid cultivar of coffee, after a culture period of 15 months, using a sequence of 3 modifications of MS medium. Vigorously growing soft, white, watery crystalline calli were obtained on MS + TIBA (1 mg/l) + L-cysteine HCl (50 mg/l) + PVP (100 mg/l). After 45 d, the calli were subcultured to MS + IAA (0.5 mg/l) + 2,4-D (0.05 mg/l) + Kn (8.6 mg/l) and maintained for the next 9 months without any transfer. On this medium, the callus proliferation was initially vigorous which slowed down after 5–6 months, and then the calli turned light brown and somewhat compact. Later, when the calli were transferred to MS + thiamine HCl (10 mg/l) + pyridoxine HCl (3 mg/l) + nicotinic acid (2 mg/l) + 2,4-D (0.2 mg/l) + 2ip (2.5 mg/l) and cultured for 2 months, they turned darker, more compact and the proliferation almost stopped. These calli were subcultured onto fresh medium of the same composition. After another 2 months of culture cream-coloured, highly friable, embryogenic calli appeared, which in turn produced a few clearly identifiable SEs in another 1 month. Further proliferation and maturation of SEs was achieved by culturing the embryogenic calli on MS + ABA (1 mg/l) for 3 months. The SEs were germinated into 2 cm tall plantlets after 2–3 subcultures, each of 2 months duration on 1/2-MS + Kn (0.1 mg/l). More... »

PAGES

670-673

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232736

DOI

http://dx.doi.org/10.1007/bf00232736

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032163542

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24194319


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