Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-07

AUTHORS

Leandro Peña, Magdalena Cervera, José Juárez, Antonio Navarro, José A. Pina, Nuria Durán-Vila, Luis Navarro

ABSTRACT

Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and ß-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus. More... »

PAGES

616-619

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232724

DOI

http://dx.doi.org/10.1007/bf00232724

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039279479

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24194307


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