Efficient regeneration of Brassica oleracea hypocotyl protoplasts and high frequency genetic transformation by direct DNA uptake View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-10

AUTHORS

Arundhati Mukhopadhyay, Reinhard Töpfer, Akshay K. Pradhan, Yaspal S. Sodhi, Hans-Henning Steinbiß, Jeff Schell, Deepak Pental

ABSTRACT

Efficient regeneration (80%) and high frequency genetic transformation (10-33%) were achieved by culturing protoplasts isolated from hypocotyl tissues of six day old Brassica oleracea seedlings and by subjecting these protoplasts to PEG mediated direct plasmid uptake. Three different plasmid vectors carrying marker genes for resistance to methotrexate (dhfr), hygromycin (hpt) and phosphinotricin (bar) were constructed and used for transformation. Large number of normal, fertile transformants were obtained with vectors carrying hpt and bar genes. No transformants could be regenerated for resistance to methotrexate as it severely suppressed shoot differentiation. More... »

PAGES

375-379

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232604

DOI

http://dx.doi.org/10.1007/bf00232604

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006527425

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24221726


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