Ultrastructural changes in suspension culture cells of Panicum maximum during cryopreservation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-05

AUTHORS

Shashi Gnanapragasam, Indra K. Vasil

ABSTRACT

A Panicum maximum cell suspension was used to study ultrastructural changes during cryopreservation. Pregrowing the cells in mannitol caused reduction in the vacuolar volume by redistribution of the large central vacuole into a number of smaller vesicles. Invaginations were formed in the plasma membrane of the cells, to accommodate the reduced cell volume. Swelling of organelles occurred during different stages of cryopreservation. The cisternae of the endoplasmic reticulum dilated and formed vesicles. Although some damage was apparent, organelles were still recognizable in cells frozen slowly and freeze-fixed at -10°C. The cells were able to repair such damage within two days in culture, and regained their normal appearance. Cells frozen slowly without any cryoprotection, and cells frozen rapidly by direct immersion into liquid nitrogen after cryoprotection, were lethally damaged by destruction of membranous structures. Osmiophilic granules were found along the plasma membrane of lethally damaged cells, indicating that their formation is a consequence of freeze damage, rather than a mechanism to prevent injury. More... »

PAGES

169-174

Journal

TITLE

Plant Cell Reports

ISSUE

4

VOLUME

11

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232526

DOI

http://dx.doi.org/10.1007/bf00232526

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036624439

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24202979


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