Shoot regeneration from stem and leaf callus of Eucalyptus tereticornis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-11

AUTHORS

M. M. Subbaiah, S. C. Minocha

ABSTRACT

Adventitious shoots were obtained from leaf and stem callus of Eucalyptus tereticornis SM. Callus was induced on B5 medium with 0.1 mg/l benzyladenine (BA) and 3 or 5 mg/l naphthalene acetic acid in the dark. Shoot initiation occurred on modified Woody Plant medium (mWP) containing 0.5 mg/l BA, 500 mg/l polyvinylpyrrolidone and 10% (v/v) coconut milk. Multiple shoots were also regenerated directly from hypocotyl segments of 4 to 6 week old seedlings on B5 medium with 0.5 mg/l BA. Regenerated shoots could be rooted with 100% efficiency on mWP medium containing 0.5 mg/l indolebutyric acid and transferred to soil in the greenhouse. Suspension cultures were obtained from the callus using B5 medium with 0.5 mg/l 2,4-dichlorophenoxyacetic acid. Callus clumps grew from less than 1 mm to 4-6 mm in diameter within two weeks on transfer to shoot regeneration medium but failed to form shoots or somatic embryos. More... »

PAGES

370-373

Journal

TITLE

Plant Cell Reports

ISSUE

7

VOLUME

9

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232401

DOI

http://dx.doi.org/10.1007/bf00232401

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051581407

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24227057


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