Induction of division and differentiation of somatic embryos in the leaf epidermis of Gaillardia picta View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-01

AUTHORS

K. G. Pillai, I. Usha Rao, I. V. R. Rao, H. Y. Mohan Ram

ABSTRACT

Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulators such as naphthaleneacetic acid or indolebutyric acid and benzylaminopurine or kinetin. Within 12 h of culture the epidermal cells showed receding of cytoplasm from the walls. After 48 h of incubation 3 or 4 localized zones, each consisting of 3-8 cells that accumulated cytoplasm and stained densely, were observed. Mitotic divisions occurred in these zones on day 3 of culture and localized masses of callus were observed in 95% of the cultures after 10 days. In another 5 days, the callus differentiated somatic embryos or roots, depending on the growth regulators and their concentration in the medium. More... »

PAGES

599-603

Journal

TITLE

Plant Cell Reports

ISSUE

12

VOLUME

10

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232378

DOI

http://dx.doi.org/10.1007/bf00232378

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048533670

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24212870


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