Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-02

AUTHORS

B. H. McCown, D. E. McCabe, D. R. Russell, D. J. Robison, K. A. Barton, K. F. Raffa

ABSTRACT

Three different target tissues (protoplast-derived cells, nodules, and stems) and two unrelated hybrid genotypes of Populus (P. alba x P. grandidentata 'Crandon' and P. nigra 'Betulifolia' x P. trichocarpa) have been stably transformed by electric discharge particle acceleration using a 18.7 kb plasmid containing NOS-NPT, CaMV 35S-GUS, and CaMV 35S-BT. Four transformed plants of one hybrid genotype, NC5339, containing all 3 genes were recovered and analyzed. Two expressed GUS and one was highly resistant to feeding by 2 lepidopteran pests (the forest tent caterpillar, Malacosoma disstria, and the gypsy moth, Lymantria dispar.) Pretreatment of the target tissues, fine-tuning of the bombardment parameters, and the use of a selection technique employing flooding of the target tissues were important for reliable recovery of transformed plants. More... »

PAGES

590-594

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232339

DOI

http://dx.doi.org/10.1007/bf00232339

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041224302

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24220719


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