Transgenic plant production mediated by Agrobacterium in Indica rice View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-06

AUTHORS

Hamid Rashid, Shuuji Yokoi, Kinya Toriyama, Kokichi Hinata

ABSTRACT

A reproducible system has been developed for the production of transgenic plants in indica rice using Agrobacterium-mediated gene transfer. Three-week-old scutella calli served as an excellent starting material. These were infected with an Agrobacterium tumefaciens strain EHA101 carrying a plasmid pIG121Hm containing genes for β-glucuronidase (GUS) and hygromycin resistnace (HygR). Hygromycin (50 mg/l) was used as a selectable agent. Inclusion of acetosyringone (50μM) in the Agrobacterium suspension and co-culture media proved to be indispensable for successful transformation. Transformation efficiency of Basmati 370 was 22% which was as high as reported in japonica rice and dicots. A large number of morphologically normal, fertile transgenic plants were obtained. Integration of foreign genes into the genome of transgenic plants was confirmed by Southern blot analysis. GUS and HygR genes were inherited and expressed in R1 progeny. Mendelian segregation was observed in some R1 progeny. More... »

PAGES

727-730

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00232216

DOI

http://dx.doi.org/10.1007/bf00232216

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044260010

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24178159


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