Transformation of grape (Vitis vinifera L.) zygotic-derived somatic embryos and regeneration of transgenic plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-06

AUTHORS

R. Scorza, J. M. Cordts, D. W. Ramming, R. L. Emershad

ABSTRACT

Transgenic grape plants were regenerated from somatic embryos derived from immature zygotic embryos of seedless grape (Vitis vinifera L.) selections. Somatic embryos were bombarded twice with 1 μm gold particles using the Biolistic PDS-1000/He device (Bio-Rad Laboratories) and then exposed to Agrobacterium tumefaciens strain C58/Z707 containing the binary plasmid pGA482GG or pCGN7314. Following cocultivation, secondary embryos were allowed to proliferate on Emershad/Ramming proliferation (ERP) medium for 6 weeks before selection on ERP medium containing 20-40 μg/ml kanamycin (kan). Transgenic embryos were identified after 3-5 months under selection and allowed to germinate and develop into rooted plants on Woody Plant Medium containing 1 μM 6-benzylaminopurine (BAP), 1.5% sucrose, 0.3% activated charcoal and 0.75% agar. Integration of the foreign genes into these grapevines was verified by growth in the presence of kan, positive GUS and PCR assays, and Southern analysis. More... »

PAGES

589-592

Journal

TITLE

Plant Cell Reports

ISSUE

9

VOLUME

14

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00231944

DOI

http://dx.doi.org/10.1007/bf00231944

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020384174

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24185603


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