Detoxification of xenobiotics by plant cells: characterisation of vacuolar amphiphilic organic anion transporters View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-12

AUTHORS

Mechteld M. A. Blake-Kalff, Julian O. D. Coleman

ABSTRACT

The transport activities of two primary ATP-dependent organic-anion transporters in the tonoplast of isolated barley (Hordeum vulgare L. cv. Klaxon) vacuoles have been characterised with N-ethylmaleimide glutathione (NEM-SG) and taurocholate as substrates. The transporters showed different sensitivities to organic anions and a variety of transport inhibitors and drugs. The vacuolar uptake of NEM-SG was inhibited by carbonylcyanide 4-trifluoromethoxyphenylhydrazone, 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS), S-(2,4-dinitrophenyl)glutathione, alkyl-S-glutathione derivatives and taurocholate but stimulated by probenecid. The uptake of taurocholate was inhibited by vinblastine, DIDS and probenecid. Both transporters were unaffected by verapamil. The kinetic properties of the transporters indicate a general preference for amphiphilic anions with some substrate overlap. These characteristics of the transporters are similar to those displayed by the multidrug resistance protein of mammalian drug-resistant cells. We suggest that these vacuolar transporters be described as plant multispecific organic anion transporters (pMOATs). More... »

PAGES

426-431

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00231398

DOI

http://dx.doi.org/10.1007/bf00231398

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006090505


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Biochemistry and Physiology Department, IACR-Rothamsted, ALS 2JQ, Harpenden, Herts., UK", 
          "id": "http://www.grid.ac/institutes/grid.418374.d", 
          "name": [
            "Biochemistry and Physiology Department, IACR-Rothamsted, ALS 2JQ, Harpenden, Herts., UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Blake-Kalff", 
        "givenName": "Mechteld M. A.", 
        "id": "sg:person.010404643237.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010404643237.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biochemistry and Physiology Department, IACR-Rothamsted, ALS 2JQ, Harpenden, Herts., UK", 
          "id": "http://www.grid.ac/institutes/grid.418374.d", 
          "name": [
            "Biochemistry and Physiology Department, IACR-Rothamsted, ALS 2JQ, Harpenden, Herts., UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Coleman", 
        "givenName": "Julian O. D.", 
        "id": "sg:person.016415011365.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016415011365.31"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/364247a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027245524", 
          "https://doi.org/10.1038/364247a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-12", 
    "datePublishedReg": "1996-12-01", 
    "description": "The transport activities of two primary ATP-dependent organic-anion transporters in the tonoplast of isolated barley (Hordeum vulgare L. cv. Klaxon) vacuoles have been characterised with N-ethylmaleimide glutathione (NEM-SG) and taurocholate as substrates. The transporters showed different sensitivities to organic anions and a variety of transport inhibitors and drugs. The vacuolar uptake of NEM-SG was inhibited by carbonylcyanide 4-trifluoromethoxyphenylhydrazone, 4,4\u2032-diisothiocyanatostilbene-2,2\u2032-disulfonic acid (DIDS), S-(2,4-dinitrophenyl)glutathione, alkyl-S-glutathione derivatives and taurocholate but stimulated by probenecid. The uptake of taurocholate was inhibited by vinblastine, DIDS and probenecid. Both transporters were unaffected by verapamil. The kinetic properties of the transporters indicate a general preference for amphiphilic anions with some substrate overlap. These characteristics of the transporters are similar to those displayed by the multidrug resistance protein of mammalian drug-resistant cells. We suggest that these vacuolar transporters be described as plant multispecific organic anion transporters (pMOATs).", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00231398", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "200"
      }
    ], 
    "keywords": [
      "amphiphilic anions", 
      "organic anion transporters", 
      "organic anions", 
      "anions", 
      "kinetic properties", 
      "drug-resistant cells", 
      "disulfonic acid", 
      "anion transporters", 
      "multidrug resistance protein", 
      "multispecific organic anion transporter", 
      "uptake of taurocholate", 
      "derivatives", 
      "taurocholate", 
      "detoxification of xenobiotics", 
      "resistance protein", 
      "vacuolar uptake", 
      "acid", 
      "characterisation", 
      "substrate", 
      "properties", 
      "transport inhibitors", 
      "transporters", 
      "uptake", 
      "cells", 
      "verapamil", 
      "probenecid", 
      "drugs", 
      "substrate overlap", 
      "vinblastine", 
      "different sensitivities", 
      "diisothiocyanatostilbene", 
      "glutathione", 
      "xenobiotics", 
      "inhibitors", 
      "transport activity", 
      "DIDS", 
      "barley vacuoles", 
      "activity", 
      "detoxification", 
      "sensitivity", 
      "variety", 
      "protein", 
      "vacuoles", 
      "plant cells", 
      "vacuolar transporters", 
      "general preference", 
      "overlap", 
      "characteristics", 
      "preferences", 
      "tonoplast"
    ], 
    "name": "Detoxification of xenobiotics by plant cells: characterisation of vacuolar amphiphilic organic anion transporters", 
    "pagination": "426-431", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1006090505"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00231398"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00231398", 
      "https://app.dimensions.ai/details/publication/pub.1006090505"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-09-02T15:48", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_287.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00231398"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00231398'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00231398'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00231398'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00231398'


 

This table displays all metadata directly associated to this object as RDF triples.

118 TRIPLES      21 PREDICATES      76 URIs      67 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00231398 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N6ed2221492dc47bdaed14f622d3f3751
4 schema:citation sg:pub.10.1038/364247a0
5 schema:datePublished 1996-12
6 schema:datePublishedReg 1996-12-01
7 schema:description The transport activities of two primary ATP-dependent organic-anion transporters in the tonoplast of isolated barley (Hordeum vulgare L. cv. Klaxon) vacuoles have been characterised with N-ethylmaleimide glutathione (NEM-SG) and taurocholate as substrates. The transporters showed different sensitivities to organic anions and a variety of transport inhibitors and drugs. The vacuolar uptake of NEM-SG was inhibited by carbonylcyanide 4-trifluoromethoxyphenylhydrazone, 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS), S-(2,4-dinitrophenyl)glutathione, alkyl-S-glutathione derivatives and taurocholate but stimulated by probenecid. The uptake of taurocholate was inhibited by vinblastine, DIDS and probenecid. Both transporters were unaffected by verapamil. The kinetic properties of the transporters indicate a general preference for amphiphilic anions with some substrate overlap. These characteristics of the transporters are similar to those displayed by the multidrug resistance protein of mammalian drug-resistant cells. We suggest that these vacuolar transporters be described as plant multispecific organic anion transporters (pMOATs).
8 schema:genre article
9 schema:isAccessibleForFree false
10 schema:isPartOf N789f833c627d431daca66d1ea7635ed2
11 N9c64b948f5fb4673a1cf98e094c08dfc
12 sg:journal.1054035
13 schema:keywords DIDS
14 acid
15 activity
16 amphiphilic anions
17 anion transporters
18 anions
19 barley vacuoles
20 cells
21 characterisation
22 characteristics
23 derivatives
24 detoxification
25 detoxification of xenobiotics
26 different sensitivities
27 diisothiocyanatostilbene
28 disulfonic acid
29 drug-resistant cells
30 drugs
31 general preference
32 glutathione
33 inhibitors
34 kinetic properties
35 multidrug resistance protein
36 multispecific organic anion transporter
37 organic anion transporters
38 organic anions
39 overlap
40 plant cells
41 preferences
42 probenecid
43 properties
44 protein
45 resistance protein
46 sensitivity
47 substrate
48 substrate overlap
49 taurocholate
50 tonoplast
51 transport activity
52 transport inhibitors
53 transporters
54 uptake
55 uptake of taurocholate
56 vacuolar transporters
57 vacuolar uptake
58 vacuoles
59 variety
60 verapamil
61 vinblastine
62 xenobiotics
63 schema:name Detoxification of xenobiotics by plant cells: characterisation of vacuolar amphiphilic organic anion transporters
64 schema:pagination 426-431
65 schema:productId N0d5d79ff9b65457f8b965f41e52f5061
66 N38a3a19d6b824a2a8d9bf8c63c443574
67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006090505
68 https://doi.org/10.1007/bf00231398
69 schema:sdDatePublished 2022-09-02T15:48
70 schema:sdLicense https://scigraph.springernature.com/explorer/license/
71 schema:sdPublisher Nae2a1f0c404b4e9f8845ae7e898ee092
72 schema:url https://doi.org/10.1007/bf00231398
73 sgo:license sg:explorer/license/
74 sgo:sdDataset articles
75 rdf:type schema:ScholarlyArticle
76 N0d5d79ff9b65457f8b965f41e52f5061 schema:name dimensions_id
77 schema:value pub.1006090505
78 rdf:type schema:PropertyValue
79 N38a3a19d6b824a2a8d9bf8c63c443574 schema:name doi
80 schema:value 10.1007/bf00231398
81 rdf:type schema:PropertyValue
82 N6ed2221492dc47bdaed14f622d3f3751 rdf:first sg:person.010404643237.96
83 rdf:rest Ne2683849d00f4cf6bf0269210547defa
84 N789f833c627d431daca66d1ea7635ed2 schema:issueNumber 4
85 rdf:type schema:PublicationIssue
86 N9c64b948f5fb4673a1cf98e094c08dfc schema:volumeNumber 200
87 rdf:type schema:PublicationVolume
88 Nae2a1f0c404b4e9f8845ae7e898ee092 schema:name Springer Nature - SN SciGraph project
89 rdf:type schema:Organization
90 Ne2683849d00f4cf6bf0269210547defa rdf:first sg:person.016415011365.31
91 rdf:rest rdf:nil
92 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
93 schema:name Biological Sciences
94 rdf:type schema:DefinedTerm
95 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
96 schema:name Biochemistry and Cell Biology
97 rdf:type schema:DefinedTerm
98 sg:journal.1054035 schema:issn 0032-0935
99 1432-2048
100 schema:name Planta
101 schema:publisher Springer Nature
102 rdf:type schema:Periodical
103 sg:person.010404643237.96 schema:affiliation grid-institutes:grid.418374.d
104 schema:familyName Blake-Kalff
105 schema:givenName Mechteld M. A.
106 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010404643237.96
107 rdf:type schema:Person
108 sg:person.016415011365.31 schema:affiliation grid-institutes:grid.418374.d
109 schema:familyName Coleman
110 schema:givenName Julian O. D.
111 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016415011365.31
112 rdf:type schema:Person
113 sg:pub.10.1038/364247a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027245524
114 https://doi.org/10.1038/364247a0
115 rdf:type schema:CreativeWork
116 grid-institutes:grid.418374.d schema:alternateName Biochemistry and Physiology Department, IACR-Rothamsted, ALS 2JQ, Harpenden, Herts., UK
117 schema:name Biochemistry and Physiology Department, IACR-Rothamsted, ALS 2JQ, Harpenden, Herts., UK
118 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...